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Literature summary for 3.4.21.B21 extracted from

  • Ishihara, K.; Miura, T.; Kuramitsu, H.K.; Okuda, K.
    Characterization of the Treponema denticola prtP gene encoding a prolyl-phenylalanine-specific protease (dentilisin) (1996), Infect. Immun., 64, 5178-5186.
    View publication on PubMedView publication on EuropePMC
Search for more literature for 3.4.21.B21

Cloned(Commentary)

Cloned (Comment) Organism
72 KDa and 43 KDa protein Treponema denticola

Inhibitors

Inhibitors Comment Organism Structure
4-mercuribenzenesulfonic acid 98% inhibition at 0.01 mM Treponema denticola
diisopropyl fluorophosphates 97% inhibition at 1 mM Treponema denticola
EDTA 41% inhibition at 5 mM Treponema denticola
Soybean trypsin inhibitor 49% inhibition at 0.01 mM Treponema denticola
ZnCl2 46% inhibition at 1 mM Treponema denticola

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
38000
-
 three bands at 72000 Da, 43000 Da and 38000 Da after boiling, SDS-PAGE Treponema denticola
43000
-
 three bands at 72000 Da, 43000 Da and 38000 Da after boiling, SDS-PAGE Treponema denticola
72000
-
 three bands at 72000 Da, 43000 Da and 38000 Da after boiling, SDS-PAGE Treponema denticola
98000
-
 two bands at 100000 Da and 98000 Da before boiling, SDS-PAGE Treponema denticola
100000
-
 two bands at 100000 Da and 98000 Da before boiling, SDS-PAGE Treponema denticola

Organism

Organism UniProt Comment Textmining
Treponema denticola
-
-
-

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
61.7
-
-
 Treponema denticola

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
alpha1-antitrypsin + H2O
-
 Treponema denticola ?
-
 ?
Fibronectin + H2O
-
 Treponema denticola ?
-
 ?
Gelatin + H2O
-
 Treponema denticola ?
-
 ?
immunogobulin G heavy chain + H2O weak substrate Treponema denticola ?
-
 ?
additional information specificity for peptide bonds containing phenylalanine and proline at the P1 and P2 positions Treponema denticola ?
-
 ?
N-succinyl-AAPL-4-nitroanilide + H2O synthetic substrate Treponema denticola ?
-
 ?
N-succinyl-AAPN-4-nitroanilide + H2O synthetic substrate Treponema denticola ?
-
 ?
Type IV collagen + H2O weak substrate Treponema denticola ?
-
 ?

Subunits

Subunits Comment Organism
polymer x * 72000 + x * 38000 + x * 43000, 72000 Da active protease associates with smaller proteins, SDS-PAGE Treponema denticola

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
8
-
-
 Treponema denticola

pI Value

Organism Comment pI Value Maximum pI Value
Treponema denticola 72000 Da and 38000 Da protein, calculated from nucleic acid sequence
-
 5.42