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Literature summary for 3.4.21.B2 extracted from

  • de Koning, P.J.; Tesselaar, K.; Bovenschen, N.; Colak, S.; Quadir, R.; Volman, T.J.; Kummer, J.A.
    The cytotoxic protease granzyme M is expressed by lymphocytes of both the innate and adaptive immune system (2010), Mol. Immunol., 47, 903-911.
    View publication on PubMed
Search for more literature for 3.4.21.B2

Cloned(Commentary)

Cloned (Comment) Organism
expressed in Pichia pastoris Homo sapiens

Organism

Organism UniProt Comment Textmining
Homo sapiens P51124
-
-

Purification (Commentary)

Purification (Comment) Organism
by cation-exchange chromatography Homo sapiens

Source Tissue

Source Tissue Comment Organism Textmining
additional information present in KHYG-1 cells, absent from Jurkat T-cells Homo sapiens
-
natural killer cell
-
 Homo sapiens
-
peripheral blood mononuclear cell
-
 Homo sapiens
-
T-lymphocyte CD4+ T cell, CD8+ T cell, natural killer T cell and gammadelta T cell Homo sapiens
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
additional information the recombinant GrM protein displays proteolytic activity only against a synthetic substrate that matches with the known cleavage specificity of the granzyme, which is after a Leu Homo sapiens ?
-
 ?

Synonyms

Synonyms Comment Organism
Granzyme M
-
 Homo sapiens
GrM
-
 Homo sapiens

Expression

Organism Comment Expression
Homo sapiens CD4+ T cells hardly express GrM (only 4%). GrM protein expression is absent in non-T non-natural killer cells in peripheral blood mononuclear cells down
Homo sapiens GrM protein expression by lymphocyte populations is not significantly affected by a panel of GrB-inducing cytokines additional information
Homo sapiens granzyme M is expressed by lymphocytes of both the innate and adaptive immune system: high expression by natural killer cells, natural killer T cells, and gammadelta T cells (70-80% of cells). CD8+ T cells also express GrM (ca. 50% of cells) and comparing the naive to early effector-memory, to late effectormemory, to effector subset, this expression gradually increases during differentiation (in particular by the differentiated effector CD27- CD45RO- subset). GrM is usually co-expressed with perforin (ca. 60% of cells). In CD8+ T cells, about 90% of GrM positive cells also express GrB up