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Literature summary for 3.4.21.B10 extracted from
- Substrate specificity of human kallikrein 6: salt and glycosaminoglycan activation effects (2006), J. Biol. Chem., 281, 3116-3126.
Activating Compound
| Activating Compound | Comment | Organism | Structure |
|---|---|---|---|
| chondroitin sulfate | activates hK6 in a concentration-dependent manner | Homo sapiens |  |
| dermatan sulfate | activates hK6 in a concentration-dependent manner | Homo sapiens | |
| heparan sulfate | activates hK6 in a concentration-dependent manner | Homo sapiens | |
| heparin | activates hK6 in a concentration-dependent manner | Homo sapiens | |
| Sodium citrate | reveals greatest activity at pH 7.5 | Homo sapiens | |
| Sodium sulfate | reveals greatest activity at pH 7.5 | Homo sapiens | |
Application
| Application | Comment | Organism |
|---|---|---|
| additional information | hK6 is involved in normal myelin turnover/demyelination processes, but it is unlikely to self-activate. Possibly it modulates ionotropic glutamate receptors and activates PAR 2 | Homo sapiens |
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
| expressed from a baculovirus/insect cell line system | Homo sapiens |
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| Ca2+ | 0.005 mM completely inhibits hK6 at pH 7.5 or 9.0 | Homo sapiens | |
| K+ | inhibits hK6 at pH 7.5 or 9.0 | Homo sapiens | |
| Mg2+ | 0.005 mM completely inhibits hK6 at pH 7.5 or 9.0 | Homo sapiens | |
| NH4+ | inhibits hK6 at pH 7.5 or 9.0 | Homo sapiens | |
| Sodium citrate | inhibits the enzyme at pH 9.0 | Homo sapiens | |
| Sodium sulfate | inhibits the enzyme at pH 9.0 | Homo sapiens | |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.0003 | - |
o-aminobenzoic acid-AFRFSQ-N-[2,4-dinitrophenyl]ethylenediamine | at pH 7.5, in the presence 2 M sodium citrate | Homo sapiens | |
| 0.0003 | - |
o-aminobenzoic acid-AFRFAQ-N-[2,4-dinitrophenyl]ethylenediamine | at pH 7.5, in the presence 2 M sodium citrate | Homo sapiens | |
| 0.001 | - |
o-aminobenzoic acid-AFRVGQ-N-[2,4-dinitrophenyl]ethylenediamine | at pH 7.5, in the presence 2 M sodium citrate | Homo sapiens | |
| 0.0011 | - |
o-aminobenzoic acid-AFRLAQ-N-[2,4-dinitrophenyl]ethylenediamine | at pH 7.5, in the presence 2 M sodium citrate | Homo sapiens | |
| 0.0014 | - |
o-aminobenzoic acid-EEHAFRFSQ-N-[2,4-dinitrophenyl]ethylenediamine | at pH 7.5, in the presence 2 M sodium citrate | Homo sapiens | |
| 0.0016 | - |
o-aminobenzoic acid-EHSAFRFAQ-N-[2,4-dinitrophenyl]ethylenediamine | at pH 7.5, in the presence 2 M sodium citrate | Homo sapiens | |
| 0.0028 | - |
o-aminobenzoic acid-KLRSSKQ-N-[2,4-dinitrophenyl]ethylenediamine | at pH 7.5, in the presence 2 M sodium citrate | Homo sapiens | |
| 0.004 | - |
o-aminobenzoic acid-KLRSSKQ-N-[2,4-dinitrophenyl]ethylenediamine | at pH 9, without salt | Homo sapiens | |
| 0.009 | - |
o-aminobenzoic acid-AFRFSQ-N-[2,4-dinitrophenyl]ethylenediamine | at pH 9, without salt | Homo sapiens | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Homo sapiens | - |
- |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
- |
Homo sapiens |
Source Tissue
| Source Tissue | Comment | Organism | Textmining |
|---|
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| alpha1-Antichymotrypsin + H2O | hydrolysis of the FRET peptide derived from alpha1-antichymotrypsin | Homo sapiens | ? | - |
? | |
| additional information | hydrolysis of the FRET peptide series o-aminobenzoic acid-XLRSSKQ-N-[2,4-dinitrophenyl]ethylenediamine, o-aminobenzoic acid-KXRSSKQ-N-[2,4-dinitrophenyl]ethylenediamine, o-aminobenzoic acid-KLRXSKQ-N-[2,4-dinitrophenyl]ethylenediamine, o-aminobenzoic acid-KLRSXKQ-N-[2,4-dinitrophenyl]ethylenediamine and FRET peptide derived from hK6 maturation/inactivation cleavage site or PAR. o-aminobenzoic acid-AFKFAQ-N-[2,4-dinitrophenyl]ethylenediamine, o-aminobenzoic acid-VLFEKKVYLQ-N-[2,4-dinitrophenyl]ethylenediamine and o-aminobenzoic acid-GTEASVVAPQ-N-[2,4-dinitrophenyl]ethylenediamine are not hydrolyzed | Homo sapiens | ? | - |
? | |
| myelin basic protein + H2O | hydrolysis of the FRET peptide derived from human myelin basic protein | Homo sapiens | ? | - |
? | |
| o-aminobenzoic acid-AFRFAQ-N-[2,4-dinitrophenyl]ethylenediamine + H2O | - |
Homo sapiens | ? | - |
? | |
| o-aminobenzoic acid-AFRFSQ-N-[2,4-dinitrophenyl]ethylenediamine + H2O | best synthetic substrate | Homo sapiens | ? | - |
? | |
| o-aminobenzoic acid-AFRLAQ-N-[2,4-dinitrophenyl]ethylenediamine + H2O | - |
Homo sapiens | ? | - |
? | |
| o-aminobenzoic acid-AFRVGQ-N-[2,4-dinitrophenyl]ethylenediamine + H2O | - |
Homo sapiens | ? | - |
? | |
| o-aminobenzoic acid-EEHAFRFSQ-N-[2,4-dinitrophenyl]ethylenediamine + H2O | - |
Homo sapiens | ? | - |
? | |
| o-aminobenzoic acid-EELAFKFAQ-N-[2,4-dinitrophenyl]ethylenediamine + H2O | - |
Homo sapiens | ? | - |
? | |
| o-aminobenzoic acid-EHSAFRFAQ-N-[2,4-dinitrophenyl]ethylenediamine + H2O | - |
Homo sapiens | ? | - |
? | |
| o-aminobenzoic acid-KLRSSKQ-N-[2,4-dinitrophenyl]ethylenediamine + H2O | - |
Homo sapiens | ? | - |
? | |
| plasminogen + H2O | hydrolysis of the FRET peptide derived from plasminogen | Homo sapiens | ? | - |
? | |
| precursor of the Abeta amyloid peptid + H2O | hydrolysis of the FRET peptide derived from human precursor of the Abeta amyloid peptide | Homo sapiens | ? | - |
? | |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| hK6 | - |
Homo sapiens |
| kallikrein 6 | - |
Homo sapiens |
Turnover Number [1/s]
| Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.4 | - |
o-aminobenzoic acid-AFRFSQ-N-[2,4-dinitrophenyl]ethylenediamine | at pH 9, without salt | Homo sapiens | |
| 2.3 | - |
o-aminobenzoic acid-KLRSSKQ-N-[2,4-dinitrophenyl]ethylenediamine | at pH 9, without salt | Homo sapiens | |
| 2.7 | - |
o-aminobenzoic acid-AFRVGQ-N-[2,4-dinitrophenyl]ethylenediamine | at pH 7.5, in the presence 2 M sodium citrate | Homo sapiens | |
| 7.1 | - |
o-aminobenzoic acid-EEHAFRFSQ-N-[2,4-dinitrophenyl]ethylenediamine | at pH 7.5, in the presence 2 M sodium citrate | Homo sapiens | |
| 7.1 | - |
o-aminobenzoic acid-AFRFAQ-N-[2,4-dinitrophenyl]ethylenediamine | at pH 7.5, in the presence 2 M sodium citrate | Homo sapiens | |
| 9.1 | - |
o-aminobenzoic acid-EHSAFRFAQ-N-[2,4-dinitrophenyl]ethylenediamine | at pH 7.5, in the presence 2 M sodium citrate | Homo sapiens | |
| 9.2 | - |
o-aminobenzoic acid-AFRLAQ-N-[2,4-dinitrophenyl]ethylenediamine | at pH 7.5, in the presence 2 M sodium citrate | Homo sapiens | |
| 11.6 | - |
o-aminobenzoic acid-AFRFSQ-N-[2,4-dinitrophenyl]ethylenediamine | at pH 7.5, in the presence 2 M sodium citrate | Homo sapiens | |
| 14.2 | - |
o-aminobenzoic acid-KLRSSKQ-N-[2,4-dinitrophenyl]ethylenediamine | at pH 7.5, in the presence 2 M sodium citrate | Homo sapiens | |
pH Range
| pH Minimum | pH Maximum | Comment | Organism |
|---|---|---|---|
| 7 | 9 | - |
Homo sapiens |
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