Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary for 3.4.21.B10 extracted from

  • Bayes, A.; Tsetsenis, T.; Ventura, S.; Vendrell, J.; Aviles, F.X.; Sotiropoulou, G.
    Human kallikrein 6 activity is regulated via an autoproteolytic mechanism of activation/inactivation (2004), Biol. Chem., 385, 517-524.
    View publication on PubMed
Search for more literature for 3.4.21.B10

Cloned(Commentary)

Cloned (Comment) Organism
the precursor form of human kallikrein 6 is overexpressed in Pichia pastoris and is autoprocessed to an active but unstable mature enzyme that subsequently yields the inactive, self-cleavage product, hK6(D81-K244) Homo sapiens

Protein Variants

Protein Variants Comment Organism
R80Q mutation stabilizes the activity of the mature enzyme Homo sapiens
S197A mutation results in complete loss of hK6 proteolytic activity Homo sapiens

Inhibitors

Inhibitors Comment Organism Structure
Bovine pancreatic trypsin inhibitor weak Homo sapiens
Bowman-Birk inhibitor weak Homo sapiens
Soybean trypsin inhibitor weak Homo sapiens

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
plasminogen + H2O Homo sapiens the enzyme is regulated by an autoactivation/autoinactivation mechanism. Mature hK67 displays a trypsin-like activity against human plasminogen, the putative physiological substrate ?
-
 ?

Organism

Organism UniProt Comment Textmining
Homo sapiens
-
-
-

Purification (Commentary)

Purification (Comment) Organism
recombinant Homo sapiens

Source Tissue

Source Tissue Comment Organism Textmining

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
Gelatin + H2O
-
 Homo sapiens ?
-
 ?
additional information hK6 does not hydrolyze the Nalpha-tosyl-L-Arg methyl ester Homo sapiens ?
-
 ?
Nalpha-benzoyl-L-Arg + H2O
-
 Homo sapiens benzoic acid + L-Arg
-
 ?
plasminogen + H2O
-
 Homo sapiens ?
-
 ?
plasminogen + H2O the enzyme is regulated by an autoactivation/autoinactivation mechanism. Mature hK67 displays a trypsin-like activity against human plasminogen, the putative physiological substrate Homo sapiens ?
-
 ?