Literature summary for 3.4.21.98 extracted from

Taremi, S.S.; Beyer, B.; Maher, M.; Yao, N.; Prosise, W.; Weber, P.C.; Malcolm, B.A.
Construction, expression, and characterization of a novel fully activated recombinant single-chain hepatitis C virus protease (1998), Protein Sci., 7, 2143-2149.

Search for more literature for 3.4.21.98

Cloned(Commentary)

Cloned (Comment)	Organism
-	Hepacivirus C

Protein Variants

Protein Variants	Comment	Organism
additional information	recombinated fused single-chain protease	Hepacivirus C

KM Value [mM]

KM Value [mM]	KM Value Maximum [mM]	Substrate	Comment	Organism	Structure
0.019	-	DTEDVVCCSMSYTWTGK	protease NS4A(21-32)-GSGS-NS3(3-181)	Hepacivirus C
0.02	-	DTEDVVCCSMSYTWTGK	protease NS3(1-631)/NS4A(1-54)	Hepacivirus C
0.08	-	DTEDVVCCSMSYTWTGK	protease NS3(1-181)/NS4A	Hepacivirus C

Organism

Organism	UniProt	Comment	Textmining
Hepacivirus C	-	-	-

Purification (Commentary)

Purification (Comment)	Organism
recombinant single-chain protease	Hepacivirus C

Storage Stability

Storage Stability	Organism
-80°C	Hepacivirus C

Substrates and Products (Substrate)

Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.	Reac.
DTEDVVCCSMSYTWTGK + H2O	-	Hepacivirus C	DTEDVVCC + SMSYTWTGK	-	?

Turnover Number [1/s]

Turnover Number Minimum [1/s]	Turnover Number Maximum [1/s]	Substrate	Comment	Organism	Structure
0.05	-	DTEDVVCCSMSYTWTGK	protease NS3(1-181)/NS4A	Hepacivirus C
0.167	-	DTEDVVCCSMSYTWTGK	protease NS3(1-631)/NS4A(1-54)	Hepacivirus C
0.317	-	DTEDVVCCSMSYTWTGK	protease NS4A(21-32)-GSGS-NS3(3-181)	Hepacivirus C

pH Optimum

pH Optimum Minimum	pH Optimum Maximum	Comment	Organism
7.5	-	protease NS4A(21-32)-GSGS-NS3(3-181)	Hepacivirus C

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Release 2023.1 (January 2023)