Literature summary for 3.4.21.98 extracted from

Breiman, A.; Vitour, D.; Vilasco, M.; Ottone, C.; Molina, S.; Pichard, L.; Fournier, C.; Delgrange, D.; Charneau, P.; Duverlie, G.; Wychowski, C.; Maurel, P.; Meurs, E.F.
A hepatitis C virus (HCV) NS3/4A protease-dependent strategy for the identification and purification of HCV-infected cells (2006), J. Gen. Virol., 87, 3587-3598.

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Application

Application	Comment	Organism
medicine	NS3/4A-mediated trans-activation assay can be used to monitor Hepatitis C virus expression in different cell lines. NS3/4A-dependent chimera can be used to monitor hepatitis C virus infection and to perform the purification of hepatitis C virus-infected cells	Hepacivirus C

Cloned(Commentary)

Cloned (Comment)	Organism
NS3 and NS34A fragments removed from HRTRIPDU3-NS3 and HRTRIPDU3(CMV)-NS3/4A, respectively, inserted into pcDNA3.1/Hygro(2)T7	Hepacivirus C

Inhibitors

Inhibitors	Comment	Organism	Structure
additional information	decrease in the efficiency of expression of NS3/4A protease, as the concentration of Hepatitis C virus-infected cells increases	Hepacivirus C

Organism

Organism	UniProt	Comment	Textmining
Hepacivirus C	-	-	-

Substrates and Products (Substrate)

Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.	Reac.
additional information	chimeric form of the Gal4VP16 transcription factor is activated in the presence of the hepatitis C virus NS3/4A protease to induce different reporter genes (choramphenical acetyltransferase, green fluorescent protein and the cell-surface marker H-2Kk) through the (Gal4)5-E1b promoter. NS5A/5B trans-cleavage motif of hepatitis C virus of genotype 1a inserted between Gal4VP16 and the N terminus of the endoplasmic reticulum-resident protein PERK is cleaved specifically by NS3/4A	Hepacivirus C	?	-	?

Synonyms

Synonyms	Comment	Organism
NS3/4A protease	-	Hepacivirus C

Cofactor

Cofactor	Comment	Organism	Structure
additional information	full NS3 protease activity requires association with NS4A	Hepacivirus C

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Release 2023.1 (January 2023)