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Literature summary for 3.4.21.98 extracted from

  • Koch, U.; Biasiol, G.; Brunetti, M.; Fattori, D.; Pallaoro, M.; Steinkühler, C.
    Role of charged residues in the catalytic mechanism of hepatitis C virus NS3 protease: electrostatic precollision guidance and transition-state stabilization (2001), Biochemistry, 40, 631-640.
    View publication on PubMed
Search for more literature for 3.4.21.98

Protein Variants

Protein Variants Comment Organism
K136M the turnover number for EAGDDIVPCSMSYTWTGA-OH is 23% of the turnover number for the wild-type enzyme, the Km-value for EAGDDIVPCSMSYTWTGA-OH is 80% of the wild-type value. The KI-value for acetyl-Asp-Glu-(diaminopropionyl)-(N-beta-dansyl)-Glu-(beta-cyclohexylalanine)-Cys-OH 7.8fold higher than the wild-type value Hepacivirus C
K136M/R109Q the turnover number for EAGDDIVPCSMSYTWTGA-OH is 12% of the turnover number for the wild-type enzyme, the Km-value for EAGDDIVPCSMSYTWTGA-OH is 2.4fold higher than the wild-type value. The KI-value for acetyl-Asp-Glu-(diaminopropionyl)-(N-beta-dansyl)-Glu-(beta-cyclohexylalanine)-Cys-OH 7.8fold higher than the wild-type value Hepacivirus C
K165S the turnover number for EAGDDIVPCSMSYTWTGA-OH is nearly identical to the turnover number for the wild-type enzyme, the Km-value for EAGDDIVPCSMSYTWTGA-OH is 1.53fold higher than the wild-type value Hepacivirus C
K165S/R161S the turnover number for EAGDDIVPCSMSYTWTGA-OH is nearly identical to the turnover number for the wild-type enzyme, the Km-value for EAGDDIVPCSMSYTWTGA-OH is 10.8fold higher than the wild-type value. The KI-value for acetyl-Asp-Glu-(diaminopropionyl)-(N-beta-dansyl)-Glu-(beta-cyclohexylalanine)-Cys-OH 4.5fold higher than the wild-type value Hepacivirus C
R109Q the turnover number for EAGDDIVPCSMSYTWTGA-OH is 60% of the turnover number for the wild-type enzyme, the Km-value for EAGDDIVPCSMSYTWTGA-OH is 3.64fold higher than the wild-type value. The KI-value for acetyl-Asp-Glu-(diaminopropionyl)-(N-beta-dansyl)-Glu-(beta-cyclohexylalanine)-Cys-OH 1.9fold higher than the wild-type value Hepacivirus C
R123T the turnover number for EAGDDIVPCSMSYTWTGA-OH is 1.2fold higher than the turnover number for the wild-type enzyme, the Km-value for EAGDDIVPCSMSYTWTGA-OH is 2.4fold higher than the wild-type value. The KI-value for acetyl-Asp-Glu-(diaminopropionyl)-(N-beta-dansyl)-Glu-(beta-cyclohexylalanine)-Cys-OH 1.5fold higher than the wild-type value Hepacivirus C
R155S the turnover number for EAGDDIVPCSMSYTWTGA-OH is 78% of the turnover number for the wild-type enzyme, the Km-value for EAGDDIVPCSMSYTWTGA-OH is 2.4fold higher than the wild-type value. The KI-value for acetyl-Asp-Glu-(diaminopropionyl)-(N-beta-dansyl)-Glu-(beta-cyclohexylalanine)-Cys-OH 3.2fold higher than the wild-type value Hepacivirus C
R161S the turnover number for EAGDDIVPCSMSYTWTGA-OH is 1.1fold higher than the turnover number for the wild-type enzyme, the Km-value for EAGDDIVPCSMSYTWTGA-OH is 2fold higher than the wild-type value. The KI-value for acetyl-Asp-Glu-(diaminopropionyl)-(N-beta-dansyl)-Glu-(beta-cyclohexylalanine)-Cys-OH 1.4fold higher than the wild-type value Hepacivirus C

Inhibitors

Inhibitors Comment Organism Structure
acetyl-Asp-Glu-(diaminopropionyl)-(N-beta-dansyl)-Glu-(beta-cyclohexylalanine)-Cys-OH
-
 Hepacivirus C

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.0004
-
 EAGDDIVPCSMSYTWTGA-OH pH 7.5, mutant enzyme K136M Hepacivirus C
0.0005
-
 EAGDDIVPCSMSYTWTGA-OH pH 7.5, mutant enzyme K165S Hepacivirus C
0.0005
-
 EAGDDIVPCSMSYTWTGA-OH pH 7.5, wild-type enzyme Hepacivirus C
0.001
-
 EAGDDIVPCSMSYTWTGA-OH pH 7.5, mutant enzyme R161S Hepacivirus C
0.0012
-
 EAGDDIVPCSMSYTWTGA-OH pH 7.5, mutant enzyme K136M/R109Q Hepacivirus C
0.0012
-
 EAGDDIVPCSMSYTWTGA-OH pH 7.5, mutant enzyme R123T Hepacivirus C
0.0012
-
 EAGDDIVPCSMSYTWTGA-OH pH 7.5, mutant enzyme R155S Hepacivirus C
0.0018
-
 EAGDDIVPCSMSYTWTGA-OH pH 7.5, mutant enzyme R109Q Hepacivirus C
0.0038
-
 EAGNDIVPCSMSYTWTGA-OH pH 7.5, wild-type enzyme Hepacivirus C
0.0041
-
 EAGDNIVPCSMSYTWTGA-OH pH 7.5, wild-type enzyme Hepacivirus C
0.0054
-
 EAGDDIVPCSMSYTWTGA-OH pH 7.5, mutant enzyme K165S/R161S Hepacivirus C
0.0252
-
 EAGNNIVPCSMSYTWTGA-OH pH 7.5, wild-type enzyme Hepacivirus C

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
hepatitis C virus polyprotein + H2O Hepacivirus C mutational cleavage ?
-
 ?

Organism

Organism UniProt Comment Textmining
Hepacivirus C
-
-
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
EAGDDIVPCSMSYTWTGA-OH + H2O
-
 Hepacivirus C EAGDDIVPC + SMSYTWTGA-OH
-
 ?
EAGDNIVPCSMSYTWTGA-OH + H2O
-
 Hepacivirus C EAGDNIVPC + SMSYTWTGA-OH
-
 ?
EAGNDIVPCSMSYTWTGA-OH + H2O
-
 Hepacivirus C EAGNDIVPC + SMSYTWTGA-OH
-
 ?
EAGNNIVPCSMSYTWTGA-OH + H2O
-
 Hepacivirus C EAGNNIVPC + SMSYTWTGA-OH
-
 ?
hepatitis C virus polyprotein + H2O mutational cleavage Hepacivirus C ?
-
 ?

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
0.083
-
 EAGDDIVPCSMSYTWTGA-OH pH 7.5, mutant enzyme K136M/R109Q Hepacivirus C
0.167
-
 EAGDDIVPCSMSYTWTGA-OH pH 7.5, mutant enzyme K136M Hepacivirus C
0.59
-
 EAGDDIVPCSMSYTWTGA-OH pH 7.5, mutant enzyme R109Q Hepacivirus C
0.67
-
 EAGNNIVPCSMSYTWTGA-OH pH 7.5, wild-type enzyme Hepacivirus C
0.7
-
 EAGDDIVPCSMSYTWTGA-OH pH 7.5, wild-type enzyme Hepacivirus C
0.7
-
 EAGNDIVPCSMSYTWTGA-OH pH 7.5, wild-type enzyme Hepacivirus C
0.72
-
 EAGDDIVPCSMSYTWTGA-OH pH 7.5, mutant enzyme K165S/R161S Hepacivirus C
0.77
-
 EAGDDIVPCSMSYTWTGA-OH pH 7.5, mutant enzyme R161S Hepacivirus C
0.78
-
 EAGDDIVPCSMSYTWTGA-OH pH 7.5, mutant enzyme R155S Hepacivirus C
0.8
-
 EAGDNIVPCSMSYTWTGA-OH pH 7.5, wild-type enzyme Hepacivirus C
0.85
-
 EAGDDIVPCSMSYTWTGA-OH pH 7.5, mutant enzyme R123T Hepacivirus C
0.95
-
 EAGDDIVPCSMSYTWTGA-OH pH 7.5, mutant enzyme K165S Hepacivirus C
6.08
-
 EAGDDIVPCSMSYTWTGA-OH pH 7.5, mutant enzyme K165S/R161S Hepacivirus C
6.08
-
 EAGDDIVPCSMSYTWTGA-OH pH 7.5, mutant enzyme R109Q Hepacivirus C
6.08
-
 EAGDDIVPCSMSYTWTGA-OH pH 7.5, mutant enzyme R155S Hepacivirus C
6.08
-
 EAGDDIVPCSMSYTWTGA-OH pH 7.5, mutant enzyme R161S Hepacivirus C
6.08
-
 EAGNNIVPCSMSYTWTGA-OH pH 7.5, wild-type enzyme Hepacivirus C

Ki Value [mM]

Ki Value [mM] Ki Value maximum [mM] Inhibitor Comment Organism Structure
0.000055
-
 acetyl-Asp-Glu-(diaminopropionyl)-(N-beta-dansyl)-Glu-(beta-cyclohexylalanine)-Cys-OH pH 7.5, mutant enzyme R161S Hepacivirus C
0.00006
-
 acetyl-Asp-Glu-(diaminopropionyl)-(N-beta-dansyl)-Glu-(beta-cyclohexylalanine)-Cys-OH pH 7.5, mutant enzyme R123T Hepacivirus C
0.0000615
-
 acetyl-Asp-Glu-(diaminopropionyl)-(N-beta-dansyl)-Glu-(beta-cyclohexylalanine)-Cys-OH pH 7.5, mutant enzyme K165S Hepacivirus C
0.000079
-
 acetyl-Asp-Glu-(diaminopropionyl)-(N-beta-dansyl)-Glu-(beta-cyclohexylalanine)-Cys-OH pH 7.5, mutant enzyme R109Q Hepacivirus C
0.000129
-
 acetyl-Asp-Glu-(diaminopropionyl)-(N-beta-dansyl)-Glu-(beta-cyclohexylalanine)-Cys-OH pH 7.5, mutant enzyme R155S Hepacivirus C
0.000179
-
 acetyl-Asp-Glu-(diaminopropionyl)-(N-beta-dansyl)-Glu-(beta-cyclohexylalanine)-Cys-OH pH 7.5, mutant enzyme K165S/R1161S Hepacivirus C
0.00031
-
 acetyl-Asp-Glu-(diaminopropionyl)-(N-beta-dansyl)-Glu-(beta-cyclohexylalanine)-Cys-OH pH 7.5, mutant enzyme K136M/R109Q Hepacivirus C
0.000313
-
 acetyl-Asp-Glu-(diaminopropionyl)-(N-beta-dansyl)-Glu-(beta-cyclohexylalanine)-Cys-OH pH 7.5, mutant enzyme K136M Hepacivirus C
0.04
-
 acetyl-Asp-Glu-(diaminopropionyl)-(N-beta-dansyl)-Glu-(beta-cyclohexylalanine)-Cys-OH pH 7.5, wild-type enzyme Hepacivirus C