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Literature summary for 3.4.21.97 extracted from

  • LaFemina, R.L.; Bakshl, K.; Long, W.J.; Pramanik, B.; Veloski, C.A.; Wolanski, B.S.; Marcy, A.L.; Hazuda, D.J.
    Characterization of a soluble stable human cytomegalovirus protease and inhibition by M-site peptide mimics (1996), J. Virol., 70, 4819-4824.
    View publication on PubMedView publication on EuropePMC
Search for more literature for 3.4.21.97

Protein Variants

Protein Variants Comment Organism
V141G/V207G soluble and stable, indistinguishable from wild-type in vitro Human betaherpesvirus 5

Inhibitors

Inhibitors Comment Organism Structure
ac-VVNASC Ki: 1.52 mM Human betaherpesvirus 5
ac-VVNASCR Ki: 0.628 mM Human betaherpesvirus 5
ac-VVNASCRL Ki: 0.390 mM Human betaherpesvirus 5
GVVNAACRLA Ki: 0.072 mM Human betaherpesvirus 5
GVVNASCRLA Ki: 0.225 mM Human betaherpesvirus 5
VVNASCRL Ki: 0.699 mM Human betaherpesvirus 5

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.414
-
 GVVNASCRLA mutant V141G Human betaherpesvirus 5
0.488
-
 GVVNASCRLA mutant A143S Human betaherpesvirus 5
0.515
-
 GVVNASCRLA wild-type enzyme Human betaherpesvirus 5
0.578
-
 GVVNASCRLA mutant V141G/V207G, soluble Human betaherpesvirus 5
0.59
-
 GVVNASCRLA mutant V141G/V207G, refolded Human betaherpesvirus 5
0.633
-
 GVVNASCRLA mutant A143G Human betaherpesvirus 5

Organism

Organism UniProt Comment Textmining
Human betaherpesvirus 5
-
-
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
GVVNASCRLA + H2O
-
 Human betaherpesvirus 5 GVVNA + SCRLA
-
 ?

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
0.182
-
 GVVNASCRLA mutant A143S Human betaherpesvirus 5
0.206
-
 GVVNASCRLA wild-type enzyme Human betaherpesvirus 5
0.22
-
 GVVNASCRLA mutant V141G/V207G, soluble Human betaherpesvirus 5
0.236
-
 GVVNASCRLA mutant V141G Human betaherpesvirus 5
0.242
-
 GVVNASCRLA mutant V141G/V207G, refolded Human betaherpesvirus 5

Ki Value [mM]

Ki Value [mM] Ki Value maximum [mM] Inhibitor Comment Organism Structure
0.072
-
 GVVNAACRLA
-
 Human betaherpesvirus 5
0.225
-
 GVVNASCRLA
-
 Human betaherpesvirus 5
0.39
-
 ac-VVNASCRL
-
 Human betaherpesvirus 5
0.628
-
 ac-VVNASCR
-
 Human betaherpesvirus 5
0.699
-
 VVNASCRL
-
 Human betaherpesvirus 5
1.52
-
 ac-VVNASC
-
 Human betaherpesvirus 5