Activating Compound | Comment | Organism | Structure |
---|---|---|---|
glycerol | promotes HCMV protease dimerization and subsequent proteolytic activity | Human betaherpesvirus 5 |
Application | Comment | Organism |
---|---|---|
analysis | cell growth selection system in the yeast Saccharomyces cerevisiae that can detect and characterize HCMV protease activity and is applicable to screen for HCMV protease inhibitors in a high-throughput format | Human betaherpesvirus 5 |
Cloned (Comment) | Organism |
---|---|
coexpression of HCMV protease with N-(5'-phosphoribosyl)anthranilate isomerase substrate in Saccharomyces cerevisiae RLY07 strain. HCMV protease expressed from the full-length GAL1 promoter, subcloned on a GAL1 vector series, with discrete protein production levels of 71%, 46%, and 16% compared to the original 100% promoter | Human betaherpesvirus 5 |
Protein Variants | Comment | Organism |
---|---|---|
S132A | is inactive | Human betaherpesvirus 5 |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
BI31 | 50% inhibitory at 0.0017 mM. Increasing inhibitor concentrations in cells expressing the active protease correlate with increasing cell proliferation | Human betaherpesvirus 5 | |
BI36 | 50% inhibitory at 0.0005 mM. Increasing inhibitor concentrations in cells expressing the active protease correlate with increasing cell proliferation | Human betaherpesvirus 5 |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Human betaherpesvirus 5 | - |
- |
- |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
N-(5'-phosphoribosyl)anthranilate isomerase + H2O | site-specific cleavage and functional inactivation of the N-(5'-phosphoribosyl)anthranilate isomerase, thereby leading to an arrest of cell proliferation. Coexpressing N-(5'-phosphoribosyl)anthranilate isomerase1194-M with the protease exhibits a growth reduction of 35%. N-(5'-phosphoribosyl)anthranilate isomerase1132-M- as well as N-(5'-phosphoribosyl)anthranilate isomerase1165-M-expressing cells do not show growth reduction upon coexpressing HCMV protease, thus no cleavage occurs. Cells expressing the modified N-(5'-phosphoribosyl)anthranilate isomerase1194-Me together with the active protease show 85% reduction of cell proliferation, is cleaved in a sequence-specific manner | Human betaherpesvirus 5 | ? | - |
? |
Synonyms | Comment | Organism |
---|---|---|
HCMV protease | - |
Human betaherpesvirus 5 |
human cytomegalovirus protease | - |
Human betaherpesvirus 5 |