Literature summary for 3.4.21.97 extracted from

Qian, C.; Lagace, L.; Massariol, M.J.; Chabot, C.; Yoakim, C.; Deziel, R.; Tong, L.
A rational approach towards successful crystallization and crystal treatment of human cytomegalovirus protease and its inhibitor complex (2000), Acta Crystallogr. Sect. D, 56, 175-180.

Search for more literature for 3.4.21.97

Crystallization (Commentary)

Crystallization (Comment)	Organism
hanging drop method, microseeding technique, each of six components in the final crystallization formula (16% polyethylene glycol 4000, 0.1 M MES pH 6.0, 0.4 M LiCl, 10% glycerol 5% tert-butanol and 5 mM Na2S2O3) plays a distinctive role and is indispensable. Free enzyme crystallizes at pH 6.0. Using 20-50 mM spermine in the crystallization buffer, crystals of two peptidomimetic inhibitor complexes with C821 are obtained at pH 7.5 and pH 8.0. Spermine is required for the inhibitor complexes to be crystallized at pH 8.0, possibly neutralizing net negative charges of the enzyme owing to its acidic pI of 5.5	Human betaherpesvirus 5

Crystallization (Comment)

Organism

hanging drop method, microseeding technique, each of six components in the final crystallization formula (16% polyethylene glycol 4000, 0.1 M MES pH 6.0, 0.4 M LiCl, 10% glycerol 5% tert-butanol and 5 mM Na2S2O3) plays a distinctive role and is indispensable. Free enzyme crystallizes at pH 6.0. Using 20-50 mM spermine in the crystallization buffer, crystals of two peptidomimetic inhibitor complexes with C821 are obtained at pH 7.5 and pH 8.0. Spermine is required for the inhibitor complexes to be crystallized at pH 8.0, possibly neutralizing net negative charges of the enzyme owing to its acidic pI of 5.5

Human betaherpesvirus 5

Organism

Organism	UniProt	Comment	Textmining
Human betaherpesvirus 5	-	-	-

pI Value

Organism	Comment	pI Value Maximum	pI Value
Human betaherpesvirus 5	isoelectric focusing, pH-gradient 5.65-4.9	-	5.3
Human betaherpesvirus 5	calculation	-	5.7

Use of this online version of BRENDA is free under the CC BY 4.0 license. See terms of use for full details.

Release 2023.1 (January 2023)