Crystallization (Comment) | Organism |
---|---|
hanging drop method, microseeding technique, each of six components in the final crystallization formula (16% polyethylene glycol 4000, 0.1 M MES pH 6.0, 0.4 M LiCl, 10% glycerol 5% tert-butanol and 5 mM Na2S2O3) plays a distinctive role and is indispensable. Free enzyme crystallizes at pH 6.0. Using 20-50 mM spermine in the crystallization buffer, crystals of two peptidomimetic inhibitor complexes with C821 are obtained at pH 7.5 and pH 8.0. Spermine is required for the inhibitor complexes to be crystallized at pH 8.0, possibly neutralizing net negative charges of the enzyme owing to its acidic pI of 5.5 | Human betaherpesvirus 5 |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Human betaherpesvirus 5 | - |
- |
- |
Organism | Comment | pI Value Maximum | pI Value |
---|---|---|---|
Human betaherpesvirus 5 | isoelectric focusing, pH-gradient 5.65-4.9 | - |
5.3 |
Human betaherpesvirus 5 | calculation | - |
5.7 |