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Literature summary for 3.4.21.96 extracted from

  • Siezen, R.J.; Bruinenberg, P.G.; Vos, P.; van Alen-Boerrigter, I.; Nijhuis, M.; Alting, A.C.; Exterkate, F.A.; de Vos, W.M.
    Engineering of the substrate-binding region of the subtilisin-like, cell-envelope proteinase of Lactococcus lactis (1993), Protein Eng., 6, 927-937.
    View publication on PubMed
Search for more literature for 3.4.21.96

Localization

Localization Comment Organism GeneOntology No. Textmining
cell envelope
-
 Lactococcus lactis 30313
-

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
145000
-
 strain SK11, x * 145000, SDS-PAGE Lactococcus lactis

Organism

Organism UniProt Comment Textmining
Lactococcus lactis
-
 SK11
-
Lactococcus lactis SK11
-
 SK11
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
alphas1-Casein fragment 1-23 + H2O
-
 Lactococcus lactis Fragments of alphas1-casein fragment 1-23
-
 ?
alphas1-Casein fragment 1-23 + H2O
-
 Lactococcus lactis SK11 Fragments of alphas1-casein fragment 1-23
-
 ?
Methoxysuccinyl-Arg-Pro-Tyr 4-nitroanilide + H2O
-
 Lactococcus lactis ?
-
 ?
Methoxysuccinyl-Arg-Pro-Tyr 4-nitroanilide + H2O
-
 Lactococcus lactis SK11 ?
-
 ?
additional information overview, specificity of wild-type SK11 proteinase and its mutants as compared to strain Wg2 Lactococcus lactis ?
-
 ?
additional information overview, specificity of wild-type SK11 proteinase and its mutants as compared to strain Wg2 Lactococcus lactis SK11 ?
-
 ?
Succinyl-Ala-Glu-Pro-Phe 4-nitroanilide + H2O
-
 Lactococcus lactis ?
-
 ?
Succinyl-Ala-Glu-Pro-Phe 4-nitroanilide + H2O
-
 Lactococcus lactis SK11 ?
-
 ?

Subunits

Subunits Comment Organism
? strain SK11, x * 145000, SDS-PAGE Lactococcus lactis
? strain SK11, autoproteolysis yields fragments of 120000, 82000, 50000 Lactococcus lactis