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Literature summary for 3.4.21.94 extracted from

  • Lee, S.N.; Kacprzak, M.M.; Day, R.; Lindberg, I.
    Processing and trafficking of a prohormone convertase 2 active site mutant (2007), Biochem. Biophys. Res. Commun., 355, 825-829.
    View publication on PubMedView publication on EuropePMC

Cloned(Commentary)

Cloned (Comment) Organism
pcDNA3 plasmid encoding either PC2 wild-type or mutant S383A expressed in CHO-K1/7B2 cells or AtT-20/7B2 cells Mus musculus

Protein Variants

Protein Variants Comment Organism
S383A active site mutant, seems to be folded correctly. Is efficiently secreted as the intact zymogen in CHO-K1 cells. Its propeptide can productively insert into the mutated binding pocket without causing misfolding. In AtT-20 cells, mutant S383A is cleaved at the secondary cleavage site within the propeptide. The mutant can not be cleaved by active PC2, so that other proprotein convertases, but not PC2, may be responsible for secondary site processing. This cleavage event is pH-dependent and is inhibited by the proprotein convertase inhibitor decanoyl-Arg-Val-Lys-Arg-chloromethylketone Mus musculus

Inhibitors

Inhibitors Comment Organism Structure
decanoyl-Arg-Val-Lys-Arg-chloromethylketone
-
Mus musculus

Organism

Organism UniProt Comment Textmining
Mus musculus
-
-
-

Purification (Commentary)

Purification (Comment) Organism
recombinant PC2 wild-type and mutant S383A purified on anion-exchange column and by gel filtration Mus musculus

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
proPC2 + H2O
-
Mus musculus mature PC2
-
?

Synonyms

Synonyms Comment Organism
PC2
-
Mus musculus
prohormone convertase 2
-
Mus musculus