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Literature summary for 3.4.21.94 extracted from

  • Johanning, K.; Juliano, M.A.; Juliano, L.; Lazure, C.; Lamango, N.S.; Steiner, D.F.; Lindberg, I.
    Specificity of prohormone convertase 2 on proenkephalin and proenkephalin-related substrates (1998), J. Biol. Chem., 273, 22672-22680.
    View publication on PubMed
Search for more literature for 3.4.21.94

Cloned(Commentary)

Cloned (Comment) Organism
overexpressed in Chinese hamster ovary cells supertransfected with rat cDNA Mus musculus

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.11
-
 CBZ-Arg-Ser-Lys-Arg-4-methylcoumarin 7-amide pH 5.0, 37°C Mus musculus
0.14
-
 Ac-Lys-Ser-Lys-Arg-4-methylcoumarin 7-amide pH 5.0, 37°C Mus musculus
0.26
-
 Ac-Arg-Pro-Lys-Arg-4-methylcoumarin 7-amide pH 5.0, 37°C Mus musculus
0.27
-
 Ac-Arg-Phe-Ala-Arg-4-methylcoumarin 7-amide pH 5.0, 37°C Mus musculus
0.33
-
 Ac-Orn-Ser-Lys-Arg-4-methylcoumarin 7-amide pH 5.0, 37°C Mus musculus
0.44
-
 Boc-Gly-Lys-Arg-4-methylcoumarin 7-amide pH 5.0, 37°C Mus musculus
0.51
-
 Boc-Gly-Arg-Arg-4-methylcoumarin 7-amide pH 5.0, 37°C Mus musculus
1.3
-
 Boc-Val-Pro-Arg-4-methylcoumarin 7-amide pH 5.0, 37°C Mus musculus

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
proenkephalin + H2O Mus musculus cleaves prohormones and proneuropeptides from inactive large precursors to generate activre peptide for extracellular release opioid-active enkephalin + ?
-
 ?

Organism

Organism UniProt Comment Textmining
Mus musculus
-
 mouse
-

Purification (Commentary)

Purification (Comment) Organism
recombinant enzyme Mus musculus

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2-aminobenzoyl-LPSDEEGESYKEVPEMEKRYGGFMQ-N-(2,4-dinitrophenyl)ethylenediamine + H2O
-
 Mus musculus 2-aminobenzoyl-LPSDEEGESYKEVPEMEKR + YGGFMQ-N-(2,4-dinitrophenyl)ethylenediamine
-
 ?
2-aminobenzoyl-Tyr-Gly-Gly-Phe-Met-Arg-Arg-Val-Gly-Arg-Pro-Glu-N-(2,4-dinitrophenyl)ethylenediamine + H2O
-
 Mus musculus ?
-
 ?
2-aminobenzoyl-Val-Pro-Arg-Met-Glu-Lys-Arg-Tyr-Gly-Gly-Phe-Met-Gln-N-(2,4-dinitrophenyl)ethylenediamine + H2O
-
 Mus musculus ?
-
 ?
Ac-Arg-Phe-Ala-Arg-4-methylcoumarin 7-amide + H2O
-
 Mus musculus Ac-Arg-Phe-Ala-Arg + 7-amino-4-methylcoumarin
-
 ?
Ac-Arg-Pro-Lys-Arg-4-methylcoumarin 7-amide + H2O
-
 Mus musculus Ac-Arg-Pro-Lys-Arg + 7-amino-4-methylcoumarin
-
 ?
Ac-Lys-Ser-Lys-Arg-4-methylcoumarin 7-amide + H2O
-
 Mus musculus Ac-Lys-Ser-Lys-Arg + 7-amino-4-methylcoumarin
-
 ?
Ac-Orn-Ser-Lys-Arg-4-methylcoumarin 7-amide + H2O
-
 Mus musculus Ac-Orn-Ser-Lys-Arg + 7-amino-4-methylcoumarin
-
 ?
Boc-Gly-Arg-Arg-4-methylcoumarin 7-amide + H2O
-
 Mus musculus Boc-Gly-Arg-Arg + 7-amino-4-methylcoumarin
-
 ?
Boc-Gly-Lys-Arg-4-methylcoumarin 7-amide + H2O
-
 Mus musculus Boc-Gly-Lys-Arg + 7-amino-4-methylcoumarin
-
 ?
Boc-Val-Pro-Arg-4-methylcoumarin 7-amide + H2O
-
 Mus musculus Boc-Val-Pro-Arg + 7-amino-4-methylcoumarin
-
 ?
CBZ-Arg-Ser-Lys-Arg-4-methylcoumarin 7-amide + H2O
-
 Mus musculus CBZ-Arg-Ser-Lys-Arg + 7-amino-4-methylcoumarin
-
 ?
additional information prefers Arg at positions P4 and P5 in peptide B IQ substrates, Boc-Val-Leu-Lys-4-methylcoumarin 7-amide is no substrate Mus musculus ?
-
 ?
pGlu-Arg-Thr-Lys-Arg-4-methylcoumarin 7-amide + H2O
-
 Mus musculus pGlu-Arg-Thr-Lys-Arg + 7-amino-4-methylcoumarin
-
 ?
proenkephalin + H2O
-
 Mus musculus opioid-active enkephalin + ?
-
 ?
proenkephalin + H2O cleaves prohormones and proneuropeptides from inactive large precursors to generate activre peptide for extracellular release Mus musculus opioid-active enkephalin + ?
-
 ?

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
0.6
-
 Ac-Arg-Phe-Ala-Arg-4-methylcoumarin 7-amide pH 5.0, 37°C Mus musculus
1.2
-
 Boc-Gly-Lys-Arg-4-methylcoumarin 7-amide pH 5.0, 37°C Mus musculus
1.8
-
 Boc-Gly-Arg-Arg-4-methylcoumarin 7-amide pH 5.0, 37°C Mus musculus
34.2
-
 pGlu-Arg-Thr-Lys-Arg-4-methylcoumarin 7-amide pH 5.0, 37°C Mus musculus
34.2
-
 CBZ-Arg-Ser-Lys-Arg-4-methylcoumarin 7-amide pH 5.0, 37°C Mus musculus
39.6
-
 Ac-Orn-Ser-Lys-Arg-4-methylcoumarin 7-amide pH 5.0, 37°C Mus musculus
43.8
-
 Ac-Lys-Ser-Lys-Arg-4-methylcoumarin 7-amide pH 5.0, 37°C Mus musculus
99
-
 Ac-Arg-Pro-Lys-Arg-4-methylcoumarin 7-amide pH 5.0, 37°C Mus musculus