Literature summary for 3.4.21.92 extracted from

Kimber, M.S.; Yu, A.Y.; Borg, M.; Leung, E.; Chan, H.S.; Houry, W.A.
Structural and theoretical studies indicate that the cylindrical protease ClpP samples extended and compact conformations (2010), Structure, 18, 798-808.

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Protein Variants

Protein Variants	Comment	Organism
A153C	the structure of a crosslinked Escherichia coli ClpP are determined in which the two heptameric rings of ClpP are held together by disulfide bonds. While all Escherichia coli ClpP structures solved to date are in the extended state, the crosslinked ClpP structure is found to be in the compact state. Under reducing condition Km (N-succinyl-Leu-Tyr-7-amido-4-methylcoumarin) is similar to wild-type but kcat is 32fold lower. Under non-reducing conditions mutant is inactive and does not bind its cognate chaperone	Escherichia coli

KM Value [mM]

KM Value [mM]	KM Value Maximum [mM]	Substrate	Comment	Organism	Structure
0.8	-	N-succinyl-Leu-Tyr-7-amido-4-methylcoumarin	wild-type, non-reducing conditions, 4°C, pH not specified in the publication	Escherichia coli
1	-	N-succinyl-Leu-Tyr-7-amido-4-methylcoumarin	wild-type, reducing conditions, 4°C, pH not specified in the publication	Escherichia coli
1.1	-	N-succinyl-Leu-Tyr-7-amido-4-methylcoumarin	mutant A153C, reducing conditions, 4°C, pH not specified in the publication	Escherichia coli

Molecular Weight [Da]

Molecular Weight [Da]	Molecular Weight Maximum [Da]	Comment	Organism
300000	-	gel filtration	Escherichia coli
346000	-	sedimentation velocity analytical ultracentrifugation, tetradecamer	Escherichia coli

Organism

Organism	UniProt	Comment	Textmining
Escherichia coli	-	-	-

Substrates and Products (Substrate)

Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.	Reac.
N-succinyl-Leu-Tyr-7-amido-4-methylcoumarin + H2O	-	Escherichia coli	N-succinyl-Leu-Tyr + 7-amino-4-methylcoumarin	-	?

Subunits

Subunits	Comment	Organism
More	tetradecamer, sedimentation velocity analytical ultracentrifugation	Escherichia coli

Synonyms

Synonyms	Comment	Organism
ClpP	-	Escherichia coli

Turnover Number [1/s]

Turnover Number Minimum [1/s]	Turnover Number Maximum [1/s]	Substrate	Comment	Organism	Structure
0.5	-	N-succinyl-Leu-Tyr-7-amido-4-methylcoumarin	mutant A153C, reducing conditions, 4°C, pH not specified in the publication	Escherichia coli
9.6	-	N-succinyl-Leu-Tyr-7-amido-4-methylcoumarin	wild-type, non-reducing conditions, 4°C, pH not specified in the publication	Escherichia coli
15.8	-	N-succinyl-Leu-Tyr-7-amido-4-methylcoumarin	wild-type, reducing conditions, 4°C, pH not specified in the publication	Escherichia coli

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Release 2023.1 (January 2023)