Crystallization (Comment) | Organism |
---|---|
SaClpP consists of two heptameric rings that form a tetradecameric barrel. Compared to other ClpP structures, the barrel is compressed by roughly 10 A along the axial direction, and the ring-ring interface contains 14 equatorial side pores that can be up to 6 A in diameter, depending on the side-chain conformations. The side pores are lined with conserved hydrophobic residues and apparently represent the long-sought exit route for peptide products from the barrel chamber | Staphylococcus aureus |
Protein Variants | Comment | Organism |
---|---|---|
E135A | mutant shows no activity | Staphylococcus aureus |
E135R | mutant shows no activity | Staphylococcus aureus |
G127 | mutant shows no activity | Staphylococcus aureus |
G128 | mutant shows no activity | Staphylococcus aureus |
G131 | mutant shows no activity | Staphylococcus aureus |
L144E | mutant shows no activity | Staphylococcus aureus |
L144G | mutant shows no activity | Staphylococcus aureus |
L144M | Km (N-succinyl-Leu-Tyr-7-amido-4-methylcoumarin): 0.523 mM, kcat: 0.003/sec | Staphylococcus aureus |
L144R | mutant shows no activity | Staphylococcus aureus |
S98A | mutant shows no activity | Staphylococcus aureus |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.52 | - |
N-succinyl-Leu-Tyr-7-amido-4-methylcoumarin | mutant L144M, pH and temperature not specified in the publication | Staphylococcus aureus | |
0.58 | - |
N-succinyl-Leu-Tyr-7-amido-4-methylcoumarin | wild-type, pH and temperature not specified in the publication | Staphylococcus aureus |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Staphylococcus aureus | - |
- |
- |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
N-succinyl-Leu-Tyr-7-amido-4-methylcoumarin + H2O | - |
Staphylococcus aureus | ? | - |
? |
Synonyms | Comment | Organism |
---|---|---|
ClpP | - |
Staphylococcus aureus |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.003 | - |
N-succinyl-Leu-Tyr-7-amido-4-methylcoumarin | mutant L144M, pH and temperature not specified in the publication | Staphylococcus aureus | |
0.093 | - |
N-succinyl-Leu-Tyr-7-amido-4-methylcoumarin | wild-type, pH and temperature not specified in the publication | Staphylococcus aureus |