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Literature summary for 3.4.21.92 extracted from

  • Geiger, S.; Boettcher, T.; Sieber, S.; Cramer, P.
    A conformational switch underlies ClpP protease function (2011), Angew. Chem. Int. Ed. Engl., 50, 5749-5752.
    View publication on PubMed

Crystallization (Commentary)

Crystallization (Comment) Organism
SaClpP consists of two heptameric rings that form a tetradecameric barrel. Compared to other ClpP structures, the barrel is compressed by roughly 10 A along the axial direction, and the ring-ring interface contains 14 equatorial side pores that can be up to 6 A in diameter, depending on the side-chain conformations. The side pores are lined with conserved hydrophobic residues and apparently represent the long-sought exit route for peptide products from the barrel chamber Staphylococcus aureus

Protein Variants

Protein Variants Comment Organism
E135A mutant shows no activity Staphylococcus aureus
E135R mutant shows no activity Staphylococcus aureus
G127 mutant shows no activity Staphylococcus aureus
G128 mutant shows no activity Staphylococcus aureus
G131 mutant shows no activity Staphylococcus aureus
L144E mutant shows no activity Staphylococcus aureus
L144G mutant shows no activity Staphylococcus aureus
L144M Km (N-succinyl-Leu-Tyr-7-amido-4-methylcoumarin): 0.523 mM, kcat: 0.003/sec Staphylococcus aureus
L144R mutant shows no activity Staphylococcus aureus
S98A mutant shows no activity Staphylococcus aureus

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.52
-
N-succinyl-Leu-Tyr-7-amido-4-methylcoumarin mutant L144M, pH and temperature not specified in the publication Staphylococcus aureus
0.58
-
N-succinyl-Leu-Tyr-7-amido-4-methylcoumarin wild-type, pH and temperature not specified in the publication Staphylococcus aureus

Organism

Organism UniProt Comment Textmining
Staphylococcus aureus
-
-
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
N-succinyl-Leu-Tyr-7-amido-4-methylcoumarin + H2O
-
Staphylococcus aureus ?
-
?

Synonyms

Synonyms Comment Organism
ClpP
-
Staphylococcus aureus

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
0.003
-
N-succinyl-Leu-Tyr-7-amido-4-methylcoumarin mutant L144M, pH and temperature not specified in the publication Staphylococcus aureus
0.093
-
N-succinyl-Leu-Tyr-7-amido-4-methylcoumarin wild-type, pH and temperature not specified in the publication Staphylococcus aureus