Cloned (Comment) | Organism |
---|---|
as His tagged fusion protein | Escherichia coli |
Protein Variants | Comment | Organism |
---|---|---|
H230A | RKH mutant to investigate the role of the RKH sequence loops | Escherichia coli |
K229A | RAH mutant to investigate the role of the RKH sequence loops | Escherichia coli |
additional information | RKH loops are important for in substrate recognition | Escherichia coli |
R228A | AKH mutant to investigate the role of the RKH sequence loops | Escherichia coli |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
CAANDENYALAA | - |
Escherichia coli | |
CAANDENYALAA-NH2 | - |
Escherichia coli |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.0005 | - |
Arc-ssrA | wild type in the presence of SspB | Escherichia coli | |
0.0012 | - |
Arc-ssrA | wild type | Escherichia coli | |
0.0024 | - |
CM-titin-ssrA | wild type | Escherichia coli | |
0.0044 | - |
Lambda O Arc | AKH mutant | Escherichia coli | |
0.011 | - |
Lambda O CM-titiin | AKH mutant | Escherichia coli | |
0.011 | - |
Lambda O CM-titiin | lambda O CM-titii | Escherichia coli | |
0.013 | - |
Lambda O Arc | wild type | Escherichia coli | |
0.02 | - |
Arc-ssrA | AKH mutant in the presence of SspB | Escherichia coli | |
0.041 | - |
Lambda O CM-titiin | wild type | Escherichia coli | |
0.041 | - |
Lambda O CM-titiin | lambda O CM-titii | Escherichia coli | |
0.08 | - |
CM-titin-ssrA | AKH mutant | Escherichia coli | |
0.14 | - |
Arc-ssrA | AKH mutant | Escherichia coli |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Escherichia coli | - |
- |
- |
Purification (Comment) | Organism |
---|---|
of the recombinant proteins by Ni2+-NTA chromatography and gel filtration, untagged protein by phenyl sepharose and Sephacryl 16/60 S-300 column chromatography | Escherichia coli |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
Arc-ssrA + H2O | Arc repressor with a C-terminal ssrA tag | Escherichia coli | peptides | - |
? | |
CM-titin-ssrA + H2O | - |
Escherichia coli | ? | - |
? | |
Lambda O Arc + H2O | Arc repressor with a N-terminal lambda O degradation tag | Escherichia coli | ? | - |
? | |
lambda O CM-titiin + H2O | - |
Escherichia coli | ? | - |
? | |
Lambda O CM-titin + H2O | - |
Escherichia coli | ? | - |
? |
Synonyms | Comment | Organism |
---|---|---|
ClpXP | ATP dependent protease | Escherichia coli |