Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary for 3.4.21.92 extracted from

  • Farrell, C.M.; Baker, T.A.; Sauer, R.T.
    Altered specificity of a AAA+ protease (2007), Mol. Cell, 25, 161-166.
    View publication on PubMedView publication on EuropePMC

Cloned(Commentary)

Cloned (Comment) Organism
as His tagged fusion protein Escherichia coli

Protein Variants

Protein Variants Comment Organism
H230A RKH mutant to investigate the role of the RKH sequence loops Escherichia coli
K229A RAH mutant to investigate the role of the RKH sequence loops Escherichia coli
additional information RKH loops are important for in substrate recognition Escherichia coli
R228A AKH mutant to investigate the role of the RKH sequence loops Escherichia coli

Inhibitors

Inhibitors Comment Organism Structure
CAANDENYALAA
-
Escherichia coli
CAANDENYALAA-NH2
-
Escherichia coli

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.0005
-
Arc-ssrA wild type in the presence of SspB Escherichia coli
0.0012
-
Arc-ssrA wild type Escherichia coli
0.0024
-
CM-titin-ssrA wild type Escherichia coli
0.0044
-
Lambda O Arc AKH mutant Escherichia coli
0.011
-
Lambda O CM-titiin AKH mutant Escherichia coli
0.011
-
Lambda O CM-titiin lambda O CM-titii Escherichia coli
0.013
-
Lambda O Arc wild type Escherichia coli
0.02
-
Arc-ssrA AKH mutant in the presence of SspB Escherichia coli
0.041
-
Lambda O CM-titiin wild type Escherichia coli
0.041
-
Lambda O CM-titiin lambda O CM-titii Escherichia coli
0.08
-
CM-titin-ssrA AKH mutant Escherichia coli
0.14
-
Arc-ssrA AKH mutant Escherichia coli

Organism

Organism UniProt Comment Textmining
Escherichia coli
-
-
-

Purification (Commentary)

Purification (Comment) Organism
of the recombinant proteins by Ni2+-NTA chromatography and gel filtration, untagged protein by phenyl sepharose and Sephacryl 16/60 S-300 column chromatography Escherichia coli

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
Arc-ssrA + H2O Arc repressor with a C-terminal ssrA tag Escherichia coli peptides
-
?
CM-titin-ssrA + H2O
-
Escherichia coli ?
-
?
Lambda O Arc + H2O Arc repressor with a N-terminal lambda O degradation tag Escherichia coli ?
-
?
lambda O CM-titiin + H2O
-
Escherichia coli ?
-
?
Lambda O CM-titin + H2O
-
Escherichia coli ?
-
?

Synonyms

Synonyms Comment Organism
ClpXP ATP dependent protease Escherichia coli