General Stability | Organism |
---|---|
Repeated freezing and thawing leads to loss of activity of subunit ClpA | Escherichia coli |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
diisopropyl fluorophosphate | inactivates ClpP | Escherichia coli |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.21 | - |
ATP | for hydrolysis of both casein and ATP | Escherichia coli |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Ca2+ | poor substitute for Mg2+ | Escherichia coli | |
Mg2+ | optimal concentration: 5.1 mM | Escherichia coli | |
Mn2+ | can partially substitute for Mg2+ | Escherichia coli |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
23000 | - |
x * 80000 (ClpA, SDS-PAGE, behaves as a dimer of MW 140000 Da on gel filtration) + x * 23000 (ClpP, SDS-PAGE, behaves as a complex of 10-12 subunits, MW 260000 Da) | Escherichia coli |
80000 | - |
x * 80000 (ClpA, SDS-PAGE, behaves as a dimer of MW 140000 Da on gel filtration) + x * 23000 (ClpP, SDS-PAGE, behaves as a complex of 10-12 subunits, MW 260000 Da) | Escherichia coli |
340000 | - |
E. coli | Escherichia coli |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Escherichia coli | - |
RGC125 (lon-) | - |
Escherichia coli RGC125 (lon-) | - |
RGC125 (lon-) | - |
Purification (Comment) | Organism |
---|---|
ClpA | Escherichia coli |
ClpP | Escherichia coli |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
additional information | - |
- |
Escherichia coli |
Storage Stability | Organism |
---|---|
-30°C, subunit ClpA, 1 month, 50% loss of activity | Escherichia coli |
-30°C, subunit ClpA, 20 mM Tris-HCl, pH 7.8, 5 mM MgCl2, 2 mM DTT, 0.5 mM EDTA, 20% v/v glycerol, 1 month, 50% loss of activity, | Escherichia coli |
4°C, 20 mM Tris-HCl, pH 7.8, 5 mM MgCl2, 0.5 mM EDTA, 10% glycerol, subunit ClpA stable for several months | Escherichia coli |
4°C, 20 mM Tris-HCl, pH 7.8, 5 mM MgCl2, 2 mM DTT, 0.5 mM EDTA, 20% v/v glycerol, 80% loss of activity after 1 week, subunit ClpA | Escherichia coli |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
casein + H2O | - |
Escherichia coli | small peptides derived from casein | - |
? | |
casein + H2O | - |
Escherichia coli RGC125 (lon-) | small peptides derived from casein | - |
? |
Subunits | Comment | Organism |
---|---|---|
? | x * 80000 (ClpA, SDS-PAGE, behaves as a dimer of MW 140000 Da on gel filtration) + x * 23000 (ClpP, SDS-PAGE, behaves as a complex of 10-12 subunits, MW 260000 Da) | Escherichia coli |
More | enzyme consists of two components: ClpP and ClpA or ClpX | Escherichia coli |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
65 | - |
10 min, 20 mM Tris-HCl, pH 7.8, 5 mM MgCl2, 0.5 mM EDTA, 10% glycerol, little or no loss of activity | Escherichia coli |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
additional information | - |
7.0 (ClpA) | Escherichia coli |
additional information | - |
8.2 (ClpP) | Escherichia coli |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
ATP | proteolytic activity | Escherichia coli | |
dATP | proteolytic activity | Escherichia coli |