Application | Comment | Organism |
---|---|---|
molecular biology | it is demonstrated that the WNV NS2B/NS3pro-hel is an active endoproteolytic enzyme at the ER membrane and the in-cell selectivity profiling of the membrane-anchored flaviviral protease is reported | West Nile virus |
Cloned (Comment) | Organism |
---|---|
expressed in Human hepatocellular carcinoma cells (Huh7 cells) | West Nile virus |
Protein Variants | Comment | Organism |
---|---|---|
S135A | inactive mutant | West Nile virus |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
West Nile virus | - |
- |
- |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
DSSTKRGGSW + H2O | each substrate plasmid contains the ER membrane-anchoring domain, a specific cleavage sequence that includes the P6-P1 P19-P49 residues and a fluorescent reporter group (DsRed) | West Nile virus | DSSTKR + GGSW | - |
? | |
KPGLKRGGAK + H2O | each substrate plasmid contains the ER membrane-anchoring domain, a specific cleavage sequence that includes the P6-P1 P19-P49 residues and a fluorescent reporter group (DsRed) | West Nile virus | KPGLKR + GGAK | - |
? | |
additional information | the substrate that corresponds to the NS4B/NS5 junction site is cleaved in trans, whereas the remaining substrates that correspond to the NS2A/NS2B,NS2B/NS3, and NS3/NS4A proprotein junction sites are poorly processed in trans under experimental conditions | West Nile virus | ? | - |
? |
Synonyms | Comment | Organism |
---|---|---|
NS2B/NS3 serine protease | - |
West Nile virus |