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Literature summary for 3.4.21.91 extracted from

  • Condotta, S.A.; Martin, M.M.; Boutin, M.; Jean, F.
    Detection and in-cell selectivity profiling of the full-length West Nile virus NS2B/NS3 serine protease using membrane-anchored fluorescent substrates (2010), Biol. Chem., 391, 549-559.
    View publication on PubMed

Application

Application Comment Organism
molecular biology it is demonstrated that the WNV NS2B/NS3pro-hel is an active endoproteolytic enzyme at the ER membrane and the in-cell selectivity profiling of the membrane-anchored flaviviral protease is reported West Nile virus

Cloned(Commentary)

Cloned (Comment) Organism
expressed in Human hepatocellular carcinoma cells (Huh7 cells) West Nile virus

Protein Variants

Protein Variants Comment Organism
S135A inactive mutant West Nile virus

Organism

Organism UniProt Comment Textmining
West Nile virus
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-
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
DSSTKRGGSW + H2O each substrate plasmid contains the ER membrane-anchoring domain, a specific cleavage sequence that includes the P6-P1 P19-P49 residues and a fluorescent reporter group (DsRed) West Nile virus DSSTKR + GGSW
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?
KPGLKRGGAK + H2O each substrate plasmid contains the ER membrane-anchoring domain, a specific cleavage sequence that includes the P6-P1 P19-P49 residues and a fluorescent reporter group (DsRed) West Nile virus KPGLKR + GGAK
-
?
additional information the substrate that corresponds to the NS4B/NS5 junction site is cleaved in trans, whereas the remaining substrates that correspond to the NS2A/NS2B,NS2B/NS3, and NS3/NS4A proprotein junction sites are poorly processed in trans under experimental conditions West Nile virus ?
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?

Synonyms

Synonyms Comment Organism
NS2B/NS3 serine protease
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West Nile virus