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Literature summary for 3.4.21.91 extracted from
- Probing the substrate specificity of the dengue virus type 2 NS3 serine protease by using internally quenched fluorescent peptides (2006), Biochem. J., 397, 203-211.
Activating Compound
| Activating Compound | Comment | Organism | Structure |
|---|---|---|---|
| additional information | presence of basic residues at the P3 and P4 positions significantly promotes reactivity | Dengue virus type 2 |
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
| NS2B(H)-NS3pro expressed in Escherichia coli | Dengue virus type 2 |
| NS2B(H)NS3pro expressed in Escherichia coli | Dengue virus type 2 |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.0045 | - |
Abz-RRRRSAG-3-nitrotyrosine-amide | - |
Dengue virus type 2 |  |
| 0.0045 | - |
Abz-RRRRSAGnY-amide | - |
Dengue virus type 2 | |
| 0.089 | - |
Abz-KKQRAGVL-3-nitrotyrosine-amide | - |
Dengue virus type 2 | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Dengue virus type 2 | - |
- |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
- |
Dengue virus type 2 |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| Abz-AAGRK-SLTLnY-amide + H2O | - |
Dengue virus type 2 | Abz-AAGRK + SLTLnY-amide | - |
? | |
| Abz-AAGRKSLTL-3-nitrotyrosine-amide + H2O | - |
Dengue virus type 2 | Abz-AAGRK + SLTL-3-nitrotyrosine-amide | - |
? | |
| Abz-AGRK-SLTnY-amide + H2O | - |
Dengue virus type 2 | Abz-AGRK + SLTnY-amide | - |
? | |
| Abz-AGRK-SnY-amide + H2O | - |
Dengue virus type 2 | Abz-AGRK + Ser-(3-nitro)Tyr-amide | - |
? | |
| Abz-AGRKGTGN-3-nitrotyrosine-amide + H2O | the poorest substrate | Dengue virus type 2 | Abz-AGRKGTGN + 3-nitrotyrosine-amide | - |
? | |
| Abz-AGRKGTGNnY-amide + H2O | - |
Dengue virus type 2 | Abz-AGRK + GTGNnY-amide | - |
? | |
| Abz-AGRKS-3-nitrotyrosine-amide + H2O | - |
Dengue virus type 2 | Abz-AGRKS + 3-nitrotyrosine-amide | - |
? | |
| Abz-AGRKSLT-3-nitrotyrosine-amide + H2O | - |
Dengue virus type 2 | Abz-AGRK + SLT-3-nitrotyrosine-amide | - |
? | |
| Abz-ARRK-SLTLnY-amide + H2O | - |
Dengue virus type 2 | Abz-ARRK + SLTLnY-amide | - |
? | |
| Abz-ARRKSLTL-3-nitrotyrosine-amide + H2O | - |
Dengue virus type 2 | Abz-ARRK + SLTL-3-nitrotyrosine-amide | - |
? | |
| Abz-FAAGRK-SLTLnY-amide + H2O | - |
Dengue virus type 2 | Abz-FAAGRK + SLTLnY-amide | - |
? | |
| Abz-FAAGRKSLTL-3-nitrotyrosine-amide + H2O | - |
Dengue virus type 2 | Abz-FAAGRK + SLTL-3-nitrotyrosine-amide | - |
? | |
| Abz-GRK-SLTLnY-amide + H2O | - |
Dengue virus type 2 | Abz-GRK + SLTLnY-amide | - |
? | |
| Abz-GRK-SnY-amide + H2O | - |
Dengue virus type 2 | Abz-GRK + Ser-(3-nitro)Tyr-amide | - |
? | |
| Abz-GRKS-3-nitrotyrosine-amide + H2O | - |
Dengue virus type 2 | Abz-GRK + S-3-nitrotyrosine-amide | - |
? | |
| Abz-GRKSLTL-3-nitrotyrosine-amide + H2O | - |
Dengue virus type 2 | Abz-GRK + SLTL-3-nitrotyrosine-amide | - |
? | |
| Abz-KKQR-AGVLnY-amide + H2O | high degree of cleavage efficiency, based on the NS2B/NS3 polyprotein cleavage site | Dengue virus type 2 | Abz-KKQR + AGVLnY-amide | - |
? | |
| Abz-KKQR-SAGMnY-amide + H2O | - |
Dengue virus type 2 | Abz-KKQR + SAGMnY-amide | - |
? | |
| Abz-KKQRAGVL-3-nitrotyrosine-amide + H2O | is based on the NS2B/NS3 polyprotein cleavage site, shows high degree of cleavage efficiency | Dengue virus type 2 | Abz-KKQR + AGVL-3-nitrotyrosine-amide | - |
? | |
| Abz-KKQRSAGM-3-nitrotyrosine-amide + H2O | substrates containing the capsid protein prime side sequence SAGM are efficiently hydrolysed | Dengue virus type 2 | Abz-KKQR + SAGM-3-nitrotyrosine-amide | - |
? | |
| Abz-NRRRR-SAGMnYamide + H2O | - |
Dengue virus type 2 | ? | - |
? | |
| Abz-NRRRRSAGM-3-nitrotyrosine-amide + H2O | - |
Dengue virus type 2 | Abz-NRRRR + SAGM-3-nitrotyrosine-amide | - |
? | |
| Abz-RK-SLTLnY-amide + H2O | - |
Dengue virus type 2 | Abz-RK + SLTLnY-amide | - |
? | |
| Abz-RK-SnY-amide + H2O | - |
Dengue virus type 2 | Abz-RK + SnY-amide | - |
? | |
| Abz-RKS-3-nitrotyrosine-amide + H2O | - |
Dengue virus type 2 | Abz-RK + S-3-nitrotyrosine-amide | - |
? | |
| Abz-RKSLTL-3-nitrotyrosine-amide + H2O | - |
Dengue virus type 2 | Abz-RK + SLTL-3-nitrotyrosine-amide | - |
? | |
| Abz-RRRR-3-nitrotyrosine-amide + H2O | marginal activity | Dengue virus type 2 | Abz-RRRR + 3-nitrotyrosine-amide | - |
? | |
| Abz-RRRR-nY-amide + H2O | - |
Dengue virus type 2 | Abz-RRRR + 3-nitro-L-tyrosine amide | - |
? | |
| Abz-RRRR-SAGMnY-amide + H2O | - |
Dengue virus type 2 | Abz-RRRR + SAGMnY-amide | - |
? | |
| Abz-RRRR-SAGnY-amide + H2O | efficient substrate of the NS3 protease, based on the Dengue virus capsid protein processing site | Dengue virus type 2 | Abz-RRRR + SAGnY-amide | - |
? | |
| Abz-RRRR-SLTLnY-amide + H2O | - |
Dengue virus type 2 | Abz-RRRR + SLTLnY-amide | - |
? | |
| Abz-RRRR-SnY-amide + H2O | - |
Dengue virus type 2 | Abz-RRRR + Ser-(3-nitro)Tyr-amide | - |
? | |
| Abz-RRRRS-3-nitrotyrosine-amide + H2O | - |
Dengue virus type 2 | Abz-RRRR + S-3-nitrotyrosine-amide | - |
? | |
| Abz-RRRRSAG-3-nitrotyrosine-amide + H2O | most efficiently hydrolysed substrate, which contains the tetrabasic non-prime side sequence RRRR of the dengue virus capsid protein | Dengue virus type 2 | Abz-RRRR + SAG-3-nitrotyrosine-amide | - |
? | |
| Abz-RRRRSAGM-3-nitrotyrosine-amide + H2O | - |
Dengue virus type 2 | Abz-RRRR + SAGM-3-nitrotyrosine-amide | - |
? | |
| Abz-RRRRSLTL-3-nitrotyrosineamide + H2O | - |
Dengue virus type 2 | Abz-RRRR + SLTL-3-nitrotyrosineamide | - |
? | |
| Abz-SKKR-AGVLnY-amide + H2O | - |
Dengue virus type 2 | Abz-SKKR + AGVLnY-amide | - |
? | |
| Abz-SKKR-SAGMnY-amide + H2O | - |
Dengue virus type 2 | Abz-SKKR + SAGMnY-amide | - |
? | |
| Abz-SKKRAGVL-3-nitrotyrosine-amide + H2O | - |
Dengue virus type 2 | Abz-SKKR + AGVL-3-nitrotyrosine-amide | - |
? | |
| Abz-SKKRSAGM-3-nitrotyrosine-amide + H2O | - |
Dengue virus type 2 | Abz-SKKR + SAGM-3-nitrotyrosine-amide | - |
? | |
| additional information | preferred substrate size for the dengue virus NS3 protease encompasses the residues from P4 to P3'. The presence of basic residues at the P3 and P4 positions significantly promotes reactivity. Serine is the preferred P1' residue | Dengue virus type 2 | ? | - |
? | |
| additional information | preferred substrate size for the Dengue virus NS3 protease encompasses the residues from P4 to P3'. Serine is the preferred P1' residue | Dengue virus type 2 | ? | - |
? | |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| dengue virus non-structural protein 3 | - |
Dengue virus type 2 |
| non-structural protein 3 serine protease | - |
Dengue virus type 2 |
| NS3 serine protease | - |
Dengue virus type 2 |
Turnover Number [1/s]
| Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.001 | - |
Abz-AGRKS-3-nitrotyrosine-amide | - |
Dengue virus type 2 | |
| 0.001 | - |
Abz-AGRK-SnY-amide | - |
Dengue virus type 2 | |
| 0.0045 | - |
Abz-SKKRAGVL-3-nitrotyrosine-amide | - |
Dengue virus type 2 | |
| 0.0045 | - |
Abz-SKKR-AGVLnY-amide | - |
Dengue virus type 2 | |
| 0.005 | - |
Abz-AGRKSLT-3-nitrotyrosine-amide | - |
Dengue virus type 2 | |
| 0.005 | - |
Abz-AGRK-SLTnY-amide | - |
Dengue virus type 2 | |
| 0.045 | - |
Abz-SKKRSAGM-3-nitrotyrosine-amide | - |
Dengue virus type 2 | |
| 0.045 | - |
Abz-SKKR-SAGMnY-amide | - |
Dengue virus type 2 | |
| 0.0967 | - |
Abz-KKQRAGVL-3-nitrotyrosine-amide | - |
Dengue virus type 2 | |
| 0.0967 | - |
Abz-KKQR-SAGMnY-amide | - |
Dengue virus type 2 | |
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