KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.001 | - |
Trypsinogen | bovine guanidinated trypsinogen | Bos taurus | |
0.007 | - |
Trypsinogen | - |
Bos taurus |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
57000 | - |
x * 57000 + x * 82000, SDS-PAGE in presence of 2-mercaptoethanol | Bos taurus |
82000 | - |
x * 57000 + x * 82000, SDS-PAGE in presence of 2-mercaptoethanol | Bos taurus |
145000 | - |
x * 145000, SDS-PAGE | Bos taurus |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Bos taurus | - |
- |
- |
Purification (Comment) | Organism |
---|---|
- |
Bos taurus |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
duodenum | - |
Bos taurus | - |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
1.36 | - |
- |
Bos taurus |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
trypsinogen + H2O | cleaved exclusively at the Lys6-Ile7-peptide bond | Bos taurus | trypsin + ? | - |
? |
Subunits | Comment | Organism |
---|---|---|
? | x * 57000 + x * 82000, SDS-PAGE in presence of 2-mercaptoethanol | Bos taurus |
? | x * 145000, SDS-PAGE | Bos taurus |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
60 | - |
complete destruction of activity towards trypsinogen after 2 min, 60% loss of esterase activity after 8 min. Presence of 4 mM CaCl2 stabilizes both activities | Bos taurus |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.005 | - |
Trypsinogen | bovine guanidinated trypsinogen | Bos taurus | |
1.48 | - |
Trypsinogen | bovine trypsinogen | Bos taurus |