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Literature summary for 3.4.21.9 extracted from

  • Anderson, L.E.; Walsh, K.A.; Neurath, H.
    Bovine enterokinase. Purification, specificity, and some molecular properties (1977), Biochemistry, 16, 3354-3360.
    View publication on PubMed

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.001
-
Trypsinogen bovine guanidinated trypsinogen Bos taurus
0.007
-
Trypsinogen
-
Bos taurus

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
57000
-
x * 57000 + x * 82000, SDS-PAGE in presence of 2-mercaptoethanol Bos taurus
82000
-
x * 57000 + x * 82000, SDS-PAGE in presence of 2-mercaptoethanol Bos taurus
145000
-
x * 145000, SDS-PAGE Bos taurus

Organism

Organism UniProt Comment Textmining
Bos taurus
-
-
-

Purification (Commentary)

Purification (Comment) Organism
-
Bos taurus

Source Tissue

Source Tissue Comment Organism Textmining
duodenum
-
Bos taurus
-

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
1.36
-
-
Bos taurus

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
trypsinogen + H2O cleaved exclusively at the Lys6-Ile7-peptide bond Bos taurus trypsin + ?
-
?

Subunits

Subunits Comment Organism
? x * 57000 + x * 82000, SDS-PAGE in presence of 2-mercaptoethanol Bos taurus
? x * 145000, SDS-PAGE Bos taurus

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
60
-
complete destruction of activity towards trypsinogen after 2 min, 60% loss of esterase activity after 8 min. Presence of 4 mM CaCl2 stabilizes both activities Bos taurus

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
0.005
-
Trypsinogen bovine guanidinated trypsinogen Bos taurus
1.48
-
Trypsinogen bovine trypsinogen Bos taurus