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Literature summary for 3.4.21.9 extracted from

  • Mikhailova, A.G.; Likhareva, V.V.; Vaskovsky, B.V.; Garanin, S.K.; Onoprienko, L.V.; Prudchenko, I.A.; Chikin, L.D.; Rumsh, L.D.
    Study of secondary specificity of enteropeptidase in comparison with trypsin (2004), Biochemistry (Moscow), 69, 909-917.
    View publication on PubMed
Search for more literature for 3.4.21.9

Application

Application Comment Organism
synthesis gene engineering studies on processing fusion proteins Bos taurus

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.125
-
 PrAD4KP26 in 0.1 M Tris-HCl buffer pH 8.0 at 37°C Bos taurus
0.16
-
 GD4KF(NO2)G in 0.1 M Tris-HCl buffer pH 8.0 at 37°C Bos taurus
0.2
-
 GD4KNfa in 0.1 M Tris-HCl buffer pH 8.0 at 37°C Bos taurus
0.437
-
 G5DKF(NO2)G in 0.1 M Tris-HCl buffer pH 8.0 at 37°C Bos taurus
1.6
-
 WDDKG in 0.1 M Tris-HCl buffer pH 8.0 at 37°C Bos taurus
2.1
-
 WDDRG in 0.1 M Tris-HCl buffer pH 8.0 at 37°C Bos taurus
3.3
-
 angiotensin II in 0.1 M Tris-HCl buffer pH 8.0 at 37°C Bos taurus
4
-
 MLTAEEKAA in 0.1 M Tris-HCl buffer pH 8.0 at 37°C Bos taurus
4.2
-
 LTAEEKA in 0.1 M Tris-HCl buffer pH 8.0 at 37°C Bos taurus

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
Trypsinogen + H2O Bos taurus
-
 ?
-
 ?

Organism

Organism UniProt Comment Textmining
Bos taurus
-
-
-

Source Tissue

Source Tissue Comment Organism Textmining
duodenum
-
 Bos taurus
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
angiotensin II + H2O
-
 Bos taurus ?
-
 ?
G5DKF(NO2)G + H2O
-
 Bos taurus ?
-
 ?
GD4KF(NO2)G + H2O
-
 Bos taurus ?
-
 ?
GD4KNfa + H2O
-
 Bos taurus ?
-
 ?
LTAEEKA + H2O Hb 2-8 Bos taurus ?
-
 ?
MLTAEEKAA + H2O Hb 1-9 Bos taurus ?
-
 ?
PrAD4KP26 + H2O fusion protein containing a modified protein A as a carrier and recoverin as a target protein Bos taurus ?
-
 ?
Trypsinogen + H2O
-
 Bos taurus ?
-
 ?
WDDKG + H2O
-
 Bos taurus ?
-
 ?
WDDRG + H2O
-
 Bos taurus ?
-
 ?

Synonyms

Synonyms Comment Organism
enterokinase
-
 Bos taurus

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
0.4
-
 angiotensin II in 0.1 M Tris-HCl buffer pH 8.0 at 37°C Bos taurus
0.49
-
 LTAEEKA in 0.1 M Tris-HCl buffer pH 8.0 at 37°C Bos taurus
0.54
-
 WDDKG in 0.1 M Tris-HCl buffer pH 8.0 at 37°C Bos taurus
0.68
-
 WDDRG in 0.1 M Tris-HCl buffer pH 8.0 at 37°C Bos taurus
0.97
-
 G5DKF(NO2)G in 0.1 M Tris-HCl buffer pH 8.0 at 37°C Bos taurus
0.97
-
 GD4KNfa in 0.1 M Tris-HCl buffer pH 8.0 at 37°C Bos taurus
0.99
-
 GD4KF(NO2)G in 0.1 M Tris-HCl buffer pH 8.0 at 37°C Bos taurus
2.62
-
 PrAD4KP26 in 0.1 M Tris-HCl buffer pH 8.0 at 37°C Bos taurus
16.67
-
 GD4KNfa in 0.1 M Tris-HCl buffer pH 8.0 at 37°C Bos taurus
17.33
-
 G5DKF(NO2)G in 0.1 M Tris-HCl buffer pH 8.0 at 37°C Bos taurus
17.83
-
 GD4KF(NO2)G in 0.1 M Tris-HCl buffer pH 8.0 at 37°C Bos taurus
25.17
-
 MLTAEEKAA in 0.1 M Tris-HCl buffer pH 8.0 at 37°C Bos taurus