Application | Comment | Organism |
---|---|---|
drug development | SPase I is a target for development of inhibitors as antibiotics | Escherichia coli |
Crystallization (Comment) | Organism |
---|---|
soluble catalytic domain of SSPase I in ternary complex with inhibitors arylomycin A2 and BAL0019193, sitting drop vapor diffusion method at 18°C, mixing of 0.002 ml of protein solution and of reservoir solution, containing 0.2 M ammonium formate, 25% PEG 2000, 0.1 M sodium cacodylate pH 6.5, and 5% tertiary-amyl alcohol, equilibration over 1 ml reservoir solution, X-ray diffraction structure determination and analysis at 2.0 A resolution, molecular replacement | Escherichia coli |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
arylomycin A2 | a lipohexapeptide-based natural product, inhibits SPase I by binding to non-overlapping subsites near the catalytic center in a noncovalent manner, binding mode, overview | Escherichia coli | |
BAL0019193 | a morpholino-beta-sultam derivative, inhibits SPase I by binding to non-overlapping subsites near the catalytic center in a noncovalent manner, binding mode, overview | Escherichia coli |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
endoplasmic reticulum | - |
Escherichia coli | 5783 | - |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Escherichia coli | P00803 | - |
- |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
additional information | SPase I is an essential membrane-bound endopeptidase with a unique Ser/Lys dyad mechanism | Escherichia coli | ? | - |
? |
Synonyms | Comment | Organism |
---|---|---|
Spase I | - |
Escherichia coli |
type I signal peptidase | - |
Escherichia coli |