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Literature summary for 3.4.21.88 extracted from

  • Smollett, K.L.; Fivian-Hughes, A.S.; Smith, J.E.; Chang, A.; Rao, T.; Davis, E.O.
    Experimental determination of translational start sites resolves uncertainties in genomic open reading frame predictions - application to Mycobacterium tuberculosis (2009), Microbiology, 155, 186-197.
    View publication on PubMedView publication on EuropePMC
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Cloned(Commentary)

Cloned (Comment) Organism
the lexA gene, including its promoter region, cloned into pEJMyc to give pKS04, giving LexA with an in-frame C-terminal Myc tag. Plasmids pKS04, pKS04mut1 (one residue deleted between the possible start codons) and the pEJMyc vector transformed separately into Mycobacterium smegmatis strain mc2155 Mycobacterium tuberculosis

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
27600
-
 Western blotting, Myc-tagged LexA Mycobacterium tuberculosis

Organism

Organism UniProt Comment Textmining
Mycobacterium tuberculosis P9WHR7
-
-
Mycobacterium tuberculosis H37Rv P9WHR7
-
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
additional information presence of LexA-Myc is present in cell-free extract from Mycobacterium tuberculosis expressing pKS04 but not pKS04mut1 or pEJMyc. The translational start site for LexA is upstream of residue 1, it is translated from a start codon 57 bp further upstream at the transcriptional start site Mycobacterium tuberculosis ?
-
 ?
additional information presence of LexA-Myc is present in cell-free extract from Mycobacterium tuberculosis expressing pKS04 but not pKS04mut1 or pEJMyc. The translational start site for LexA is upstream of residue 1, it is translated from a start codon 57 bp further upstream at the transcriptional start site Mycobacterium tuberculosis H37Rv ?
-
 ?

Synonyms

Synonyms Comment Organism
LexA
-
 Mycobacterium tuberculosis