Any feedback?
Literature summary for 3.4.21.84 extracted from
- Further studies on lipopolysaccharide-sensitive serine protease zymogen (factor C): its isolation from Limulus polyphemus hemocytes and identification as an intracellular zymogen activated by alpha-chymotrypsin, not by trypsin (1991), J. Biochem., 109, 150-157.
Activating Compound
| Activating Compound | Comment | Organism | Structure |
|---|---|---|---|
| additional information | the zymogen factor C shows autocatalytic activation in presence of bacterial lipopolysaccharide | Limulus polyphemus | |
| additional information | the zymogen factor C shows autocatalytic activation in presence of bacterial lipopolysaccharide | Tachypleus tridentatus |
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| D-Phe-Pro-Arg chloromethyl ketone | - |
Limulus polyphemus |  |
| D-Phe-Pro-Arg chloromethyl ketone | - |
Tachypleus tridentatus | |
| DFP | - |
Limulus polyphemus | |
| DFP | - |
Tachypleus tridentatus | |
| leupeptin | weak | Limulus polyphemus | |
| leupeptin | weak | Tachypleus tridentatus | |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| additional information | - |
additional information | - |
Tachypleus tridentatus | |
| 0.026 | - |
tert-butoxycarbonyl-Asp(O-benzyl)-Pro-Arg-7-amido-4-methylcoumarin | - |
Tachypleus tridentatus | |
| 0.039 | - |
Tert-Butoxycarbonyl-Asp(O-benzyl)-Ala-Arg 4-methylcoumarin 7-amide | - |
Tachypleus tridentatus | |
| 0.045 | - |
tert-butoxycarbonyl-Lys(benzyloxycarbonyl)-Gly-Arg 4-methylcoumarin 7-amide | - |
Tachypleus tridentatus | |
| 0.054 | - |
N-tert-Butoxycarbonyl-Val-Pro-Arg 4-nitroanilide | - |
Tachypleus tridentatus | |
| 0.066 | - |
Tert-Butoxycarbonyl-Gln-Pro-Arg 4-methylcoumarin 7-amide | - |
Tachypleus tridentatus | |
| 0.088 | - |
Tert-Butoxycarbonyl-Met-Ala-Arg 4-methylcoumarin 7-amide | - |
Tachypleus tridentatus | |
| 0.114 | - |
Tert-Butoxycarbonyl-Val-Pro-Arg 4-methylcoumarin 7-amide | - |
Tachypleus tridentatus | |
| 0.19 | - |
tert-butoxycarbonyl-Ala-Pro-Arg-7-amido-4-methylcoumarin | - |
Tachypleus tridentatus |
Localization
| Localization | Comment | Organism | GeneOntology No. | Textmining |
|---|---|---|---|---|
| intracellular | - |
Limulus polyphemus | 5622 | - |
| intracellular | - |
Tachypleus tridentatus | 5622 | - |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 43000 | - |
x * 80000 + x * 43000, Limulus polyphemus, SDS-PAGE after reduction with 2-mercaptoethanol | Limulus polyphemus |
| 80000 | - |
x * 80000 + x * 43000, Limulus polyphemus, SDS-PAGE after reduction with 2-mercaptoethanol | Limulus polyphemus |
| 123000 | - |
x * 123000, Limulus polyphemus, SDS-PAGE under nonreducing conditions | Limulus polyphemus |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| additional information | Limulus polyphemus | factor C is an initiator in the hemolymph coagulation system of horseshoe crab (Limulus polyphemus, Tachypleus tridentatus) | ? | - |
? | |
| additional information | Tachypleus tridentatus | factor C is an initiator in the hemolymph coagulation system of horseshoe crab (Limulus polyphemus, Tachypleus tridentatus) | ? | - |
? | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Limulus polyphemus | - |
- |
- |
| Tachypleus tridentatus | - |
- |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
- |
Limulus polyphemus |
Source Tissue
| Source Tissue | Comment | Organism | Textmining |
|---|---|---|---|
| hemocyte | - |
Limulus polyphemus | - |
| hemocyte | - |
Tachypleus tridentatus | - |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| additional information | alpha-thrombin like specificity towards synthetic tripeptide substrates | Limulus polyphemus | ? | - |
? | |
| additional information | alpha-thrombin like specificity towards synthetic tripeptide substrates | Tachypleus tridentatus | ? | - |
? | |
| additional information | factor C is an initiator in the hemolymph coagulation system of horseshoe crab (Limulus polyphemus, Tachypleus tridentatus) | Limulus polyphemus | ? | - |
? | |
| additional information | factor C is an initiator in the hemolymph coagulation system of horseshoe crab (Limulus polyphemus, Tachypleus tridentatus) | Tachypleus tridentatus | ? | - |
? | |
| N-tert-Butoxycarbonyl-Asp(O-benzyl)-Pro-Arg 4-methylcoumarin 7-amide + H2O | - |
Limulus polyphemus | ? | - |
? | |
| N-tert-Butoxycarbonyl-Asp(O-benzyl)-Pro-Arg 4-methylcoumarin 7-amide + H2O | - |
Tachypleus tridentatus | ? | - |
? | |
| N-tert-Butoxycarbonyl-Val-Pro-Arg 4-nitroanilide + H2O | - |
Limulus polyphemus | ? | - |
? | |
| N-tert-Butoxycarbonyl-Val-Pro-Arg 4-nitroanilide + H2O | - |
Tachypleus tridentatus | ? | - |
? | |
| tert-butoxycarbonyl-Ala-Pro-Arg-7-amido-4-methylcoumarin + H2O | - |
Tachypleus tridentatus | tert-butoxycarbonyl-Ala-Pro-Arg + 7-amino-4-methylcoumarin | - |
? | |
| tert-butoxycarbonyl-Asp(O-benzyl)-Ala-Arg 4-methylcoumarin 7-amide + H2O | - |
Limulus polyphemus | ? | - |
? | |
| tert-butoxycarbonyl-Asp(O-benzyl)-Ala-Arg 4-methylcoumarin 7-amide + H2O | - |
Tachypleus tridentatus | ? | - |
? | |
| tert-butoxycarbonyl-Asp(O-benzyl)-Pro-Arg-7-amido-4-methylcoumarin + H2O | - |
Tachypleus tridentatus | tert-butoxycarbonyl-Asp(O-benzyl)-Pro-Arg + 7-amino-4-methylcoumarin | - |
? | |
| tert-butoxycarbonyl-Asp-Pro-Arg 4-methylcoumarin 7-amide + H2O | - |
Limulus polyphemus | ? | - |
? | |
| tert-butoxycarbonyl-Asp-Pro-Arg 4-methylcoumarin 7-amide + H2O | - |
Tachypleus tridentatus | ? | - |
? | |
| tert-butoxycarbonyl-Gln-Pro-Arg 4-methylcoumarin 7-amide + H2O | - |
Limulus polyphemus | ? | - |
? | |
| tert-butoxycarbonyl-Gln-Pro-Arg 4-methylcoumarin 7-amide + H2O | - |
Tachypleus tridentatus | ? | - |
? | |
| tert-butoxycarbonyl-Glu(O-benzyl)-Gly-Arg 4-methylcoumarin 7-amide + H2O | - |
Limulus polyphemus | ? | - |
? | |
| tert-butoxycarbonyl-Glu(O-benzyl)-Gly-Arg 4-methylcoumarin 7-amide + H2O | - |
Tachypleus tridentatus | ? | - |
? | |
| tert-butoxycarbonyl-Gly-Ala-Arg-4-methylcoumarin 7-amide + H2O | - |
Limulus polyphemus | ? | - |
? | |
| tert-butoxycarbonyl-Gly-Ala-Arg-4-methylcoumarin 7-amide + H2O | - |
Tachypleus tridentatus | ? | - |
? | |
| tert-butoxycarbonyl-Gly-Pro-Arg 4-methylcoumarin 7-amide + H2O | - |
Limulus polyphemus | ? | - |
? | |
| tert-butoxycarbonyl-Gly-Pro-Arg 4-methylcoumarin 7-amide + H2O | - |
Tachypleus tridentatus | ? | - |
? | |
| tert-butoxycarbonyl-Ile-Gly-Arg 4-methylcoumarin 7-amide + H2O | - |
Limulus polyphemus | ? | - |
? | |
| tert-butoxycarbonyl-Ile-Gly-Arg 4-methylcoumarin 7-amide + H2O | - |
Tachypleus tridentatus | ? | - |
? | |
| Tert-Butoxycarbonyl-Lys(benzyloxycarbonyl)-Gly-Arg-4-methylcoumarin 7-amide + H2O | - |
Limulus polyphemus | ? | - |
? | |
| Tert-Butoxycarbonyl-Lys(benzyloxycarbonyl)-Gly-Arg-4-methylcoumarin 7-amide + H2O | - |
Tachypleus tridentatus | ? | - |
? | |
| Tert-Butoxycarbonyl-Lys-Gly-Arg 4-methylcoumaryl 7-amide + H2O | - |
Limulus polyphemus | ? | - |
? | |
| Tert-Butoxycarbonyl-Lys-Gly-Arg 4-methylcoumaryl 7-amide + H2O | - |
Tachypleus tridentatus | ? | - |
? | |
| Tert-Butoxycarbonyl-Met-Ala-Arg 4-methylcoumarin 7-amide + H2O | - |
Limulus polyphemus | ? | - |
? | |
| Tert-Butoxycarbonyl-Met-Ala-Arg 4-methylcoumarin 7-amide + H2O | - |
Tachypleus tridentatus | ? | - |
? | |
| Tert-Butoxycarbonyl-Ser(O-benzyl)-Ala-Arg 4-methylcoumarin 7-amide + H2O | - |
Limulus polyphemus | ? | - |
? | |
| Tert-Butoxycarbonyl-Ser(O-benzyl)-Ala-Arg 4-methylcoumarin 7-amide + H2O | - |
Tachypleus tridentatus | ? | - |
? | |
| Tert-Butoxycarbonyl-Val-Gly-Arg 4-methylcoumarin 7-amide + H2O | - |
Limulus polyphemus | ? | - |
? | |
| Tert-Butoxycarbonyl-Val-Gly-Arg 4-methylcoumarin 7-amide + H2O | - |
Tachypleus tridentatus | ? | - |
? | |
| Tert-Butoxycarbonyl-Val-Pro-Arg 4-methylcoumarin 7-amide + H2O | - |
Limulus polyphemus | ? | - |
? | |
| Tert-Butoxycarbonyl-Val-Pro-Arg 4-methylcoumarin 7-amide + H2O | - |
Tachypleus tridentatus | ? | - |
? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| ? | x * 123000, Limulus polyphemus, SDS-PAGE under nonreducing conditions | Limulus polyphemus |
| ? | x * 80000 + x * 43000, Limulus polyphemus, SDS-PAGE after reduction with 2-mercaptoethanol | Limulus polyphemus |
Turnover Number [1/s]
| Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| additional information | - |
additional information | - |
Tachypleus tridentatus | |
| 10 | - |
tert-butoxycarbonyl-Lys(benzyloxycarbonyl)-Gly-Arg 4-methylcoumarin 7-amide | - |
Tachypleus tridentatus | |
| 18 | - |
Tert-Butoxycarbonyl-Asp(O-benzyl)-Ala-Arg 4-methylcoumarin 7-amide | - |
Tachypleus tridentatus | |
| 27 | - |
Tert-Butoxycarbonyl-Met-Ala-Arg 4-methylcoumarin 7-amide | - |
Tachypleus tridentatus | |
| 34 | - |
Tert-Butoxycarbonyl-Gln-Pro-Arg 4-methylcoumarin 7-amide | - |
Tachypleus tridentatus | |
| 48 | - |
Tert-Butoxycarbonyl-Val-Pro-Arg 4-methylcoumarin 7-amide | - |
Tachypleus tridentatus | |
| 72 | - |
tert-butoxycarbonyl-Ala-Pro-Arg-7-amido-4-methylcoumarin | - |
Tachypleus tridentatus | |
| 75 | - |
tert-butoxycarbonyl-Asp(O-benzyl)-Pro-Arg-7-amido-4-methylcoumarin | - |
Tachypleus tridentatus | |
| 391 | - |
N-tert-Butoxycarbonyl-Val-Pro-Arg 4-nitroanilide | - |
Tachypleus tridentatus | |
Use of this online version of BRENDA is free under the CC BY 4.0 license. See terms of use for full details.
Release 2023.1 (January 2023)
Legal
Curated at
Member of
