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Literature summary for 3.4.21.83 extracted from
- Possible role of inter-domain salt bridges in oligopeptidase B from Trypanosoma brucei: critical role of Glu172 of non-catalytic beta-propeller domain in catalytic activity and Glu490 of catalytic domain in stability of OPB (2013), J. Biochem., 154, 465-473.
Protein Variants
| Protein Variants | Comment | Organism |
|---|---|---|
| E172Q | mutation in residue predicted to form interdomain salt bridges, residue is critical for catalytic activity | Trypanosoma brucei |
| E490Q | mutation in residue predicted to form interdomain salt bridges. Mutant exhibits markedly decreased thermal stability. The boundary regions between the beta-propeller domain and catalytic domains of the E490Q mutant are unfolded with heat treatment | Trypanosoma brucei |
| E524Q | mutation in residue predicted to form interdomain salt bridges | Trypanosoma brucei |
| R689A | mutation in residue predicted to form interdomain salt bridges | Trypanosoma brucei |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.00017 | - |
N2-benzyloxycarbonyl-Arg-Arg-7-amido-4-methylcoumarin | mutant E524Q, pH not specified in the publication, temperature not specified in the publication | Trypanosoma brucei |  |
| 0.00027 | - |
N2-benzyloxycarbonyl-Arg-Arg-7-amido-4-methylcoumarin | wild-type, pH not specified in the publication, temperature not specified in the publication | Trypanosoma brucei | |
| 0.00029 | - |
benzyloxycarbonyl-Arg-Thr-Lys-Arg-7-amido-4-methylcoumarin | mutant E524Q, pH not specified in the publication, temperature not specified in the publication | Trypanosoma brucei | |
| 0.00036 | - |
benzyloxycarbonyl-Leu-Lys-Arg-7-amido-4-methylcoumarin | mutant E524Q, pH not specified in the publication, temperature not specified in the publication | Trypanosoma brucei | |
| 0.00043 | - |
N2-benzyloxycarbonyl-Arg-Arg-7-amido-4-methylcoumarin | mutant E490Q, pH not specified in the publication, temperature not specified in the publication | Trypanosoma brucei | |
| 0.00063 | - |
benzyloxycarbonyl-Arg-Thr-Lys-Arg-7-amido-4-methylcoumarin | wild-type, pH not specified in the publication, temperature not specified in the publication | Trypanosoma brucei | |
| 0.00078 | - |
benzyloxycarbonyl-Arg-Thr-Lys-Arg-7-amido-4-methylcoumarin | mutant R689A, pH not specified in the publication, temperature not specified in the publication | Trypanosoma brucei | |
| 0.0011 | - |
benzyloxycarbonyl-Arg-Thr-Lys-Arg-7-amido-4-methylcoumarin | mutant E490Q, pH not specified in the publication, temperature not specified in the publication | Trypanosoma brucei | |
| 0.00117 | - |
benzyloxycarbonyl-Leu-Lys-Arg-7-amido-4-methylcoumarin | wild-type, pH not specified in the publication, temperature not specified in the publication | Trypanosoma brucei | |
| 0.00268 | - |
benzoyl-Arg-7-amido-4-methylcoumarin | wild-type, pH not specified in the publication, temperature not specified in the publication | Trypanosoma brucei | |
| 0.00334 | - |
benzoyloxycarbonyl-Phe-Arg-7-amido-4-methylcoumarin | wild-type, pH not specified in the publication, temperature not specified in the publication | Trypanosoma brucei | |
| 0.00365 | - |
benzyloxycarbonyl-Leu-Lys-Arg-7-amido-4-methylcoumarin | mutant E490Q, pH not specified in the publication, temperature not specified in the publication | Trypanosoma brucei | |
| 0.00421 | - |
benzoyloxycarbonyl-Phe-Arg-7-amido-4-methylcoumarin | mutant E524Q, pH not specified in the publication, temperature not specified in the publication | Trypanosoma brucei | |
| 0.00493 | - |
N2-benzyloxycarbonyl-Arg-Arg-7-amido-4-methylcoumarin | mutant R689A, pH not specified in the publication, temperature not specified in the publication | Trypanosoma brucei | |
| 0.00564 | - |
benzoyl-Arg-7-amido-4-methylcoumarin | mutant E524Q, pH not specified in the publication, temperature not specified in the publication | Trypanosoma brucei | |
| 0.00596 | - |
N2-benzyloxycarbonyl-Arg-Arg-7-amido-4-methylcoumarin | mutant E172Q, pH not specified in the publication, temperature not specified in the publication | Trypanosoma brucei | |
| 0.00846 | - |
benzoyl-Arg-7-amido-4-methylcoumarin | mutant E490Q, pH not specified in the publication, temperature not specified in the publication | Trypanosoma brucei | |
| 0.00856 | - |
benzyloxycarbonyl-Leu-Lys-Arg-7-amido-4-methylcoumarin | mutant R689A, pH not specified in the publication, temperature not specified in the publication | Trypanosoma brucei | |
| 0.0106 | - |
benzyloxycarbonyl-Arg-Thr-Lys-Arg-7-amido-4-methylcoumarin | mutant E172Q, pH not specified in the publication, temperature not specified in the publication | Trypanosoma brucei | |
| 0.0108 | - |
benzoyloxycarbonyl-Phe-Arg-7-amido-4-methylcoumarin | mutant E490Q, pH not specified in the publication, temperature not specified in the publication | Trypanosoma brucei | |
| 0.0177 | - |
benzyloxycarbonyl-Leu-Lys-Arg-7-amido-4-methylcoumarin | mutant E172Q, pH not specified in the publication, temperature not specified in the publication | Trypanosoma brucei | |
| 0.0185 | - |
benzoyloxycarbonyl-Phe-Arg-7-amido-4-methylcoumarin | mutant R689A, pH not specified in the publication, temperature not specified in the publication | Trypanosoma brucei | |
| 0.0212 | - |
benzoyl-Arg-7-amido-4-methylcoumarin | mutant E172Q, pH not specified in the publication, temperature not specified in the publication | Trypanosoma brucei | |
| 0.034 | - |
benzoyloxycarbonyl-Phe-Arg-7-amido-4-methylcoumarin | mutant E172Q, pH not specified in the publication, temperature not specified in the publication | Trypanosoma brucei | |
| 0.0411 | - |
benzoyl-Arg-7-amido-4-methylcoumarin | mutant R689A, pH not specified in the publication, temperature not specified in the publication | Trypanosoma brucei | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Trypanosoma brucei | - |
- |
- |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| benzoyl-Arg-7-amido-4-methylcoumarin + H2O | - |
Trypanosoma brucei | benzoyl-Arg + 7-amino-4-methylcoumarin | - |
? | |
| benzyloxycarbonyl-Arg-Arg-7-amido-4-methylcoumarin + H2O | - |
Trypanosoma brucei | benzyloxycarbonyl-Arg-Arg + 7-amino-4-methylcoumarin | - |
? | |
| benzyloxycarbonyl-Arg-Thr-Lys-Arg-7-amido-4-methylcoumarin + H2O | - |
Trypanosoma brucei | benzyloxycarbonyl-Arg-Thr-Lys-Arg + 7-amino-4-methylcoumarin | - |
? | |
| benzyloxycarbonyl-Leu-Lys-Arg-7-amido-4-methylcoumarin + H2O | - |
Trypanosoma brucei | benzyloxycarbonyl-Leu-Lys-Arg + 7-amino-4-methylcoumarin | - |
? | |
| benzyloxycarbonyl-Phe-Arg-7-amido-4-methylcoumarin + H2O | - |
Trypanosoma brucei | benzyloxycarbonyl-Phe-Arg + 7-amino-4-methylcoumarin | - |
? | |
Temperature Stability [°C]
| Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 42 | - |
30 min, wild-type, 89% residual activity, mutants E172Q, E490Q, E524Q and R689A 99%, 9%, 49% and 73% residual activity, respectively | Trypanosoma brucei |
Turnover Number [1/s]
| Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 1.17 | - |
benzyloxycarbonyl-Leu-Lys-Arg-7-amido-4-methylcoumarin | mutant E172Q, pH not specified in the publication, temperature not specified in the publication | Trypanosoma brucei | |
| 1.38 | - |
benzyloxycarbonyl-Arg-Thr-Lys-Arg-7-amido-4-methylcoumarin | mutant E172Q, pH not specified in the publication, temperature not specified in the publication | Trypanosoma brucei | |
| 2.91 | - |
benzyloxycarbonyl-Leu-Lys-Arg-7-amido-4-methylcoumarin | mutant R689A, pH not specified in the publication, temperature not specified in the publication | Trypanosoma brucei | |
| 3.36 | - |
benzyloxycarbonyl-Leu-Lys-Arg-7-amido-4-methylcoumarin | wild-type, pH not specified in the publication, temperature not specified in the publication | Trypanosoma brucei | |
| 3.62 | - |
benzoyloxycarbonyl-Phe-Arg-7-amido-4-methylcoumarin | mutant R689A, pH not specified in the publication, temperature not specified in the publication | Trypanosoma brucei | |
| 3.91 | - |
benzoyloxycarbonyl-Phe-Arg-7-amido-4-methylcoumarin | mutant E172Q, pH not specified in the publication, temperature not specified in the publication | Trypanosoma brucei | |
| 4.25 | - |
benzyloxycarbonyl-Arg-Thr-Lys-Arg-7-amido-4-methylcoumarin | mutant R689A, pH not specified in the publication, temperature not specified in the publication | Trypanosoma brucei | |
| 5.16 | - |
benzyloxycarbonyl-Arg-Thr-Lys-Arg-7-amido-4-methylcoumarin | wild-type, pH not specified in the publication, temperature not specified in the publication | Trypanosoma brucei | |
| 5.82 | - |
N2-benzyloxycarbonyl-Arg-Arg-7-amido-4-methylcoumarin | wild-type, pH not specified in the publication, temperature not specified in the publication | Trypanosoma brucei | |
| 6.61 | - |
benzoyl-Arg-7-amido-4-methylcoumarin | mutant E172Q, pH not specified in the publication, temperature not specified in the publication | Trypanosoma brucei | |
| 6.64 | - |
benzyloxycarbonyl-Arg-Thr-Lys-Arg-7-amido-4-methylcoumarin | mutant E524Q, pH not specified in the publication, temperature not specified in the publication | Trypanosoma brucei | |
| 6.93 | - |
benzyloxycarbonyl-Leu-Lys-Arg-7-amido-4-methylcoumarin | mutant E524Q, pH not specified in the publication, temperature not specified in the publication | Trypanosoma brucei | |
| 7 | - |
benzoyloxycarbonyl-Phe-Arg-7-amido-4-methylcoumarin | mutant E524Q, pH not specified in the publication, temperature not specified in the publication | Trypanosoma brucei | |
| 8.29 | - |
benzoyloxycarbonyl-Phe-Arg-7-amido-4-methylcoumarin | wild-type, pH not specified in the publication, temperature not specified in the publication | Trypanosoma brucei | |
| 8.72 | - |
N2-benzyloxycarbonyl-Arg-Arg-7-amido-4-methylcoumarin | mutant E172Q, pH not specified in the publication, temperature not specified in the publication | Trypanosoma brucei | |
| 9.55 | - |
benzoyloxycarbonyl-Phe-Arg-7-amido-4-methylcoumarin | mutant E490Q, pH not specified in the publication, temperature not specified in the publication | Trypanosoma brucei | |
| 10 | - |
benzyloxycarbonyl-Arg-Thr-Lys-Arg-7-amido-4-methylcoumarin | mutant E490Q, pH not specified in the publication, temperature not specified in the publication | Trypanosoma brucei | |
| 11.4 | - |
N2-benzyloxycarbonyl-Arg-Arg-7-amido-4-methylcoumarin | mutant R689A, pH not specified in the publication, temperature not specified in the publication | Trypanosoma brucei | |
| 11.8 | - |
N2-benzyloxycarbonyl-Arg-Arg-7-amido-4-methylcoumarin | mutant E524Q, pH not specified in the publication, temperature not specified in the publication | Trypanosoma brucei | |
| 12.3 | - |
benzyloxycarbonyl-Leu-Lys-Arg-7-amido-4-methylcoumarin | mutant E490Q, pH not specified in the publication, temperature not specified in the publication | Trypanosoma brucei | |
| 13.3 | - |
benzoyl-Arg-7-amido-4-methylcoumarin | wild-type, pH not specified in the publication, temperature not specified in the publication | Trypanosoma brucei | |
| 13.4 | - |
benzoyl-Arg-7-amido-4-methylcoumarin | mutant R689A, pH not specified in the publication, temperature not specified in the publication | Trypanosoma brucei | |
| 14.3 | - |
N2-benzyloxycarbonyl-Arg-Arg-7-amido-4-methylcoumarin | mutant E490Q, pH not specified in the publication, temperature not specified in the publication | Trypanosoma brucei | |
| 22.8 | - |
benzoyl-Arg-7-amido-4-methylcoumarin | mutant E524Q, pH not specified in the publication, temperature not specified in the publication | Trypanosoma brucei | |
| 33.9 | - |
benzoyl-Arg-7-amido-4-methylcoumarin | mutant E490Q, pH not specified in the publication, temperature not specified in the publication | Trypanosoma brucei | |
kcat/KM [mM/s]
| kcat/KM Value [1/mMs-1] | kcat/KM Value Maximum [1/mMs-1] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 31 | - |
benzyloxycarbonyl-Arg-Thr-Lys-Arg-7-amido-4-methylcoumarin | mutant E172Q, pH not specified in the publication, temperature not specified in the publication | Trypanosoma brucei | |
| 66 | - |
benzyloxycarbonyl-Leu-Lys-Arg-7-amido-4-methylcoumarin | mutant E172Q, pH not specified in the publication, temperature not specified in the publication | Trypanosoma brucei | |
| 120 | - |
benzoyloxycarbonyl-Phe-Arg-7-amido-4-methylcoumarin | mutant E172Q, pH not specified in the publication, temperature not specified in the publication | Trypanosoma brucei | |
| 190 | - |
benzoyloxycarbonyl-Phe-Arg-7-amido-4-methylcoumarin | mutant R689A, pH not specified in the publication, temperature not specified in the publication | Trypanosoma brucei | |
| 310 | - |
benzoyl-Arg-7-amido-4-methylcoumarin | mutant E172Q, pH not specified in the publication, temperature not specified in the publication | Trypanosoma brucei | |
| 330 | - |
benzoyl-Arg-7-amido-4-methylcoumarin | mutant R689A, pH not specified in the publication, temperature not specified in the publication | Trypanosoma brucei | |
| 340 | - |
benzyloxycarbonyl-Leu-Lys-Arg-7-amido-4-methylcoumarin | mutant R689A, pH not specified in the publication, temperature not specified in the publication | Trypanosoma brucei | |
| 880 | - |
benzoyloxycarbonyl-Phe-Arg-7-amido-4-methylcoumarin | mutant E490Q, pH not specified in the publication, temperature not specified in the publication | Trypanosoma brucei | |
| 1460 | - |
N2-benzyloxycarbonyl-Arg-Arg-7-amido-4-methylcoumarin | mutant E172Q, pH not specified in the publication, temperature not specified in the publication | Trypanosoma brucei | |
| 1660 | - |
benzoyloxycarbonyl-Phe-Arg-7-amido-4-methylcoumarin | mutant E524Q, pH not specified in the publication, temperature not specified in the publication | Trypanosoma brucei | |
| 2310 | - |
N2-benzyloxycarbonyl-Arg-Arg-7-amido-4-methylcoumarin | mutant R689A, pH not specified in the publication, temperature not specified in the publication | Trypanosoma brucei | |
| 2480 | - |
benzoyloxycarbonyl-Phe-Arg-7-amido-4-methylcoumarin | wild-type, pH not specified in the publication, temperature not specified in the publication | Trypanosoma brucei | |
| 2870 | - |
benzyloxycarbonyl-Leu-Lys-Arg-7-amido-4-methylcoumarin | wild-type, pH not specified in the publication, temperature not specified in the publication | Trypanosoma brucei | |
| 3370 | - |
benzyloxycarbonyl-Leu-Lys-Arg-7-amido-4-methylcoumarin | mutant E490Q, pH not specified in the publication, temperature not specified in the publication | Trypanosoma brucei | |
| 4010 | - |
benzoyl-Arg-7-amido-4-methylcoumarin | mutant E490Q, pH not specified in the publication, temperature not specified in the publication | Trypanosoma brucei | |
| 4040 | - |
benzoyl-Arg-7-amido-4-methylcoumarin | mutant E524Q, pH not specified in the publication, temperature not specified in the publication | Trypanosoma brucei | |
| 4960 | - |
benzoyl-Arg-7-amido-4-methylcoumarin | wild-type, pH not specified in the publication, temperature not specified in the publication | Trypanosoma brucei | |
| 5450 | - |
benzyloxycarbonyl-Arg-Thr-Lys-Arg-7-amido-4-methylcoumarin | mutant R689A, pH not specified in the publication, temperature not specified in the publication | Trypanosoma brucei | |
| 8910 | - |
benzyloxycarbonyl-Arg-Thr-Lys-Arg-7-amido-4-methylcoumarin | wild-type, pH not specified in the publication, temperature not specified in the publication | Trypanosoma brucei | |
| 9090 | - |
benzyloxycarbonyl-Arg-Thr-Lys-Arg-7-amido-4-methylcoumarin | mutant E490Q, pH not specified in the publication, temperature not specified in the publication | Trypanosoma brucei | |
| 19300 | - |
benzyloxycarbonyl-Leu-Lys-Arg-7-amido-4-methylcoumarin | mutant E524Q, pH not specified in the publication, temperature not specified in the publication | Trypanosoma brucei | |
| 21600 | - |
N2-benzyloxycarbonyl-Arg-Arg-7-amido-4-methylcoumarin | wild-type, pH not specified in the publication, temperature not specified in the publication | Trypanosoma brucei | |
| 22900 | - |
benzyloxycarbonyl-Arg-Thr-Lys-Arg-7-amido-4-methylcoumarin | mutant E524Q, pH not specified in the publication, temperature not specified in the publication | Trypanosoma brucei | |
| 33300 | - |
N2-benzyloxycarbonyl-Arg-Arg-7-amido-4-methylcoumarin | mutant E490Q, pH not specified in the publication, temperature not specified in the publication | Trypanosoma brucei | |
| 69400 | - |
N2-benzyloxycarbonyl-Arg-Arg-7-amido-4-methylcoumarin | mutant E524Q, pH not specified in the publication, temperature not specified in the publication | Trypanosoma brucei | |
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