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Literature summary for 3.4.21.83 extracted from
- Protease II from Escherichia coli. Purification and characterization (1975), J. Biol. Chem., 250, 7771-7779.
General Stability
| General Stability | Organism |
|---|---|
| Ca2+ decreases stability | Escherichia coli |
| Repeated freezing and thawing does not affect esterase activity | Escherichia coli |
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| Aromatic amidines | - |
Escherichia coli | |
| benzamidine | - |
Escherichia coli |  |
| Co2+ | - |
Escherichia coli | |
| Fe2+ | - |
Escherichia coli | |
| Hg2+ | - |
Escherichia coli | |
| L-arginine | - |
Escherichia coli | |
| additional information | 1,10-phenanthroline; EDTA; not: chelating agents; PMSF; several natural trypsin inhibitors; sulfhydryl agents | Escherichia coli | |
| p-aminobenzamidine | - |
Escherichia coli | |
| Zn2+ | - |
Escherichia coli | |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.47 | - |
tosyl-Lys-methyl ester | N-benzoyl-L-Arg ethyl ester | Escherichia coli | |
Localization
| Localization | Comment | Organism | GeneOntology No. | Textmining |
|---|---|---|---|---|
| cytoplasm | - |
Escherichia coli | 5737 | - |
Metals/Ions
| Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|
| Ca2+ | stimulates amidase and proteolytic activities, maximal activation of 25% at concentrations above 25 mM | Escherichia coli | |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 58000 | - |
E. coli, sucrose density gradient centrifugation, gel filtration | Escherichia coli |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Escherichia coli | - |
- |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
- |
Escherichia coli |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| additional information | - |
- |
Escherichia coli |
Storage Stability
| Storage Stability | Organism |
|---|---|
| -80°C, 5 weeks, without or with 25 and 50 mM CaCl2, the remaining activities are 88%, 50% and 32% respectively | Escherichia coli |
| -80°C, maximal stability in dilute solutions at protein concentrations no less than 0.3 mg/ml, pH 7.8 | Escherichia coli |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| azocasein + H2O | - |
Escherichia coli | ? | - |
? | |
| Denatured casein + H2O | weak | Escherichia coli | ? | - |
? | |
| L-Phe-L-Arg 2-naphthylamide + H2O | - |
Escherichia coli | ? | - |
? | |
| N-Benzoyl-DL-Arg 4-nitroanilide + H2O | - |
Escherichia coli | N-Benzoyl-DL-Arg + 4-nitroaniline | - |
? | |
| N-Benzoyl-L-Arg ethyl ester + H2O | - |
Escherichia coli | N-Benzoyl-L-Arg + ethanol | - |
? | |
| N-carbobenzoxy-L-Lys 4-nitrophenyl ester + H2O | - |
Escherichia coli | N-carbobenzoxy-L-Lys + 4-nitrophenol | - |
? | |
| Tosyl-Lys methyl ester + H2O | - |
Escherichia coli | Tosyl-Lys + benzyl alcohol | - |
? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| monomer | 1 * 58000, SDS-PAGE | Escherichia coli |
Temperature Stability [°C]
| Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 40 | - |
3 h, stable | Escherichia coli |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 7.5 | 9 | N-benzoyl-DL-arginine 4-nitroanilide | Escherichia coli |
| 7.6 | 8.9 | azocasein | Escherichia coli |
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