Literature summary for 3.4.21.79 extracted from

Yang, J.; Pemberton, A.; Morrison, W.; Connelley, T.
Granzyme B is an essential mediator in CD8 T cell killing of theileria parva-infected cells (2019), Infect. Immun., 87, e00386-18 .

Search for more literature for 3.4.21.79

Cloned(Commentary)

Cloned (Comment)	Organism
recombinant expression of functional and nonfunctional forms of bovine granzyme B in COS-7 cells. Establishment of an enzymatic assay to detect and quantify the expression of functional granzyme B protein. Using this assay, the levels of killing of different Theileria parva-specific CD8+ T-cell clones are found to be significantly correlated with the levels of granzyme B protein but not the levels of mRNA transcript expression	Bos taurus

Cloned (Comment)

Organism

recombinant expression of functional and nonfunctional forms of bovine granzyme B in COS-7 cells. Establishment of an enzymatic assay to detect and quantify the expression of functional granzyme B protein. Using this assay, the levels of killing of different Theileria parva-specific CD8+ T-cell clones are found to be significantly correlated with the levels of granzyme B protein but not the levels of mRNA transcript expression

Bos taurus

Inhibitors

Inhibitors	Comment	Organism
acetyl-Ile-Ile-Glu-Pro-Asp-aldehyde	granzyme B-specific inhibitor	Bos taurus
additional information	no inhibition by benzyloxycarbonyl-Val-Ala-Asp-(O-methyl)-fluoromethylketone	Bos taurus
N-benzyloxycarbonyl-Ile-Glu(-O-methyl)-Thr-Asp(-O-methyl)-fluoromethylketone	i.e. Z-IETD-FMK, specifically and effectively blocks the activity of cattle granzyme B and inhibits the killing of target cells by bovine CD8+ T cells	Bos taurus

Organism

Organism	UniProt	Comment	Textmining
Bos taurus	F1MTF4	-	-
Bos taurus Holstein-Friesian	F1MTF4	-	-

Source Tissue

Source Tissue	Comment	Organism	Textmining
T-lymphocyte	CD8+ T-cell	Bos taurus	-

Substrates and Products (Substrate)

Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.
acetyl-Ile-Glu-Pro-Asp-4-nitroanilide + H2O	-	Bos taurus	acetyl-Ile-Glu-Pro-Asp + 4-nitroaniline	-	?
acetyl-Ile-Glu-Pro-Asp-4-nitroanilide + H2O	-	Bos taurus Holstein-Friesian	acetyl-Ile-Glu-Pro-Asp + 4-nitroaniline	-	?
additional information	no activity with succinyl-Gly-Gly-Phe-4-nitroanilide	Bos taurus	?	-	?
additional information	no activity with succinyl-Gly-Gly-Phe-4-nitroanilide	Bos taurus Holstein-Friesian	?	-	?

Temperature Optimum [°C]

Temperature Optimum [°C]	Temperature Optimum Maximum [°C]	Comment	Organism
37	-	assay at	Bos taurus

pH Optimum

pH Optimum Minimum	pH Optimum Maximum	Comment	Organism
7	-	assay at	Bos taurus

General Information

General Information	Comment	Organism
additional information	establishment of an enzymatic assay to detect and quantify the expression of functional granzyme B protein. Using this assay, the levels of killing of different Theileria parva-specific CD8+ T-cell clones are found to be significantly correlated with the levels of granzyme B protein but not the levels of mRNA transcript expression. Using inhibitors specific for perforin and granzyme B confirms that CD8+ T-cell killing of parasitized cells is dependent on granule exocytosis and, specifically, granzyme B	Bos taurus
physiological function	cytotoxic CD8+ T-cells are important mediators of immunity against the bovine intracellular protozoan parasite Theileria parva. Granzyme B is an essential mediator in CD8 T-cell killing of Theileria parva-infected cells. CD8+ T-cell killing of parasitized cells is dependent on granule exocytosis and, specifically, granzyme B. The granzyme B-mediated death of parasitized cells is independent of caspases. Granzyme B activates the proapoptotic molecule Bid. The cytotoxic activity of T-cells is not dependent on caspases but is associated with activation of Bid	Bos taurus