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Literature summary for 3.4.21.79 extracted from

  • Li, X.; Zhang, G.; An, G.; Liu, S.; Lai, Y.
    Expression, purification and anticancer analysis of GST-tagged human perforin and granzyme B proteins in human laryngeal cancer Hep-2 cells (2014), Protein Expr. Purif., 95, 38-43.
    View publication on PubMed
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Cloned(Commentary)

Cloned (Comment) Organism
functional recombinant expression of GST-tagged enzyme in Escherichia coli strain BL21(DE3) Homo sapiens

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
61000
-
 x * 61000, recombinant GST-tagged enzyme , SDS-OPAGE Homo sapiens

Organism

Organism UniProt Comment Textmining
Homo sapiens P10144
-
-

Purification (Commentary)

Purification (Comment) Organism
recombinant GST-tagged enzyme from Escherichia coli strain BL21(DE3) by ultracentrifugation, glutathione affinity chromatography and dialysis to 97% purity Homo sapiens

Source Tissue

Source Tissue Comment Organism Textmining
HEp-2 cell
-
 Homo sapiens
-
laryngeal cancer cell
-
 Homo sapiens
-

Subunits

Subunits Comment Organism
? x * 61000, recombinant GST-tagged enzyme , SDS-OPAGE Homo sapiens

General Information

General Information Comment Organism
physiological function the recombinant and native enzyme directly inhibits the growth of human laryngeal cancer Hep-2 cells in vitro and in vivo, respectively Homo sapiens