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Literature summary for 3.4.21.79 extracted from

  • Boivin, W.A.; Shackleford, M.; Vanden Hoek, A.; Zhao, H.; Hackett, T.L.; Knight, D.A.; Granville, D.J.
    Granzyme B cleaves decorin, biglycan and soluble betaglycan, releasing active transforming growth factor-beta1 (2012), PLoS ONE, 7, e33163.
    View publication on PubMedView publication on EuropePMC

Localization

Localization Comment Organism GeneOntology No. Textmining
extracellular
-
Homo sapiens
-
-

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
beta-glycan + H2O Homo sapiens
-
?
-
?
biglycan + H2O Homo sapiens
-
?
-
?
decorin + H2O Homo sapiens
-
?
-
?
fibrillin-1 + H2O Homo sapiens
-
?
-
?

Organism

Organism UniProt Comment Textmining
Homo sapiens P10144
-
-

Source Tissue

Source Tissue Comment Organism Textmining
commercial preparation
-
Homo sapiens
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
beta-glycan + H2O
-
Homo sapiens ?
-
?
beta-glycan + H2O 100 kDa protein, cleavage by the enzyme into fragments of about 60 kDa and 40 kDa Homo sapiens ?
-
?
biglycan + H2O
-
Homo sapiens ?
-
?
biglycan + H2O 40 kDa protein, cleavage by the enzyme into fragments of about 30 kDa and 20 kDa Homo sapiens ?
-
?
decorin + H2O
-
Homo sapiens ?
-
?
decorin + H2O 65 kDa protein, cleavage by the enzyme into fragments of about 50 kDa and 30 kDa Homo sapiens ?
-
?
fibrillin-1 + H2O
-
Homo sapiens ?
-
?
additional information the enzyme cleaves exracellular matrix proteins, release of active TGF-beta1 Homo sapiens ?
-
?

Synonyms

Synonyms Comment Organism
GrB
-
Homo sapiens
pro-apoptotic serine protease
-
Homo sapiens

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
37
-
assay at Homo sapiens

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7.4
-
assay at Homo sapiens

Expression

Organism Comment Expression
Homo sapiens extracellular levels of the enzyme are elevated in the bodily fluids in chronic inflammatory diseases such as atherosclerosis and rheumatoid arthritis up

General Information

General Information Comment Organism
physiological function pathogenic extracellular role for GrB in cardiovascular disease, during inflammation, the enzyme accumulates in the extracellular space, retains its activity, and is capable of cleaving extracellular matrix proteins Homo sapiens

kcat/KM [mM/s]

kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
1
-
decorin pH 7.4, 37°C Homo sapiens
1.7
-
biglycan pH 7.4, 37°C Homo sapiens
5.9
-
beta-glycan pH 7.4, 37°C Homo sapiens