Literature summary for 3.4.21.79 extracted from

Gehrmann, M.; Doss, B.T.; Wagner, M.; Zettlitz, K.A.; Kontermann, R.E.; Foulds, G.; Pockley, A.G.; Multhoff, G.
A novel expression and purification system for the production of enzymatic and biologically active human granzyme B (2011), J. Immunol. Methods, 371, 8-17.

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Application

Application	Comment	Organism
medicine	internalization of grB by membrane Hsp70 positive tumor cells is dependent on mammalian glycosylation of GrB. Neuraminic acid blocks binding of GrB to CT26 tumor cells	Homo sapiens
synthesis	expression system for the production of high yields of enzymatic and biologically active human grB by transfection of HEK-293 with grB. The HEK-293 host cells are protected from apoptotic cell death by fusing an inactivation site coupled to a (His)6 tag to the gene sequence of GrB. Inactive grB which is actively released from HEK-293 cells by insertion of a Igkappa leader sequence is purified on a nickel column utilizing the (His)6 tag. After enterokinase digestion and heparin affinity chromatography, high yields of enzymatic and biologically active human grB are obtained	Homo sapiens

Cloned(Commentary)

Cloned (Comment)	Organism
expression in HEK-293 cells	Homo sapiens

Protein Variants

Protein Variants	Comment	Organism
additional information	expression system for the production of high yields of enzymatic and biologically active human grB by transfection of HEK-293 with grB. The HEK-293 host cells are protected from apoptotic cell death by fusing an inactivation site coupled to a (His)6 tag to the gene sequence of GrB. Inactive grB which is actively released from HEK-293 cells by insertion of a Igkappa leader sequence is purified on a nickel column utilizing the (His)6 tag. After enterokinase digestion and heparin affinity chromatography, high yields of enzymatic and biologically active human grB are obtained	Homo sapiens

Organism

Organism	UniProt	Comment	Textmining
Homo sapiens	-	-	-

Substrates and Products (Substrate)

Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.	Reac.
N-acetyl-L-Ile-L-Glu-L-Pro-L-Asp-4-nitroanilide + H2O	-	Homo sapiens	?	-	?

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Release 2023.1 (January 2023)