Application | Comment | Organism |
---|---|---|
degradation | human and murine GzmB are distinct enzymes with different substrate preferences. Subtle differences in enzyme structure can radically affect substrate selection. Caspases are essential for apoptosis initiated by mouse GzmB | Mus musculus |
degradation | human and murine GzmB are distinct enzymes with different substrate preferences. Subtle differences in enzyme structure can radically affect substrate selection. Caspases are not essential for apoptosis initiated by human GzmB | Homo sapiens |
Cloned (Comment) | Organism |
---|---|
expressed in Pichia pastoris | Homo sapiens |
into vector pPIC6alpha and expressed in Pichia pastoris X-33 cells | Mus musculus |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
additional information | inhibition of caspases does not clonogenically rescue cells from human GzmB | Homo sapiens | |
additional information | inhibition of caspases may clonogenically rescue cells from mouse GzmB | Mus musculus |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Homo sapiens | - |
- |
- |
Mus musculus | - |
- |
- |
Purification (Comment) | Organism |
---|---|
by nickel affinity chromatography | Mus musculus |
by nickel affinity chromatography | Homo sapiens |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
acetyl-IETD-7-amido-4-methylcoumarin + H2O | - |
Mus musculus | acetyl-IETD + 7-amino-4-methylcoumarin | - |
? | |
acetyl-IETD-7-amido-4-methylcoumarin + H2O | - |
Homo sapiens | acetyl-IETD + 7-amino-4-methylcoumarin | - |
? | |
BID + H2O | both human and mouse BID are cleaved efficiently | Homo sapiens | truncated BID + ? | - |
? | |
caspase-8 + H2O | cleaves human caspase-8 but fails to cleave the mouse counterpart of caspase-8. Mouse caspase-8 is processed only upon addition of cytochrome c and dATP to J774 extracts to activate the apoptosome pathway to caspase activation | Homo sapiens | ? | - |
? | |
ICAD + H2O | cleaves human ICAD but fails to cleave the mouse counterpart | Homo sapiens | ? | - |
? | |
additional information | BID is a poor substrate for mouse GzmB. Fails to cleave caspase-8 and ICAD from either human or murine origin. Cleaves nucleophosmin of human origin very inefficiently, does not cleave nucleophosmin within J774 cell free extracts | Mus musculus | ? | - |
? | |
nucleophosmin + H2O | efficiently cleaves nucleophosmin of human origin, does not cleave nucleophosmin within J774 cell free extracts | Homo sapiens | ? | - |
? | |
pro-caspase-3 + H2O | activates caspase-3, whether of human or murine origin, with a similar efficiency | Mus musculus | ? | - |
? | |
pro-caspase-3 + H2O | activates caspase-3, whether of human or murine origin, with a similar efficiency | Homo sapiens | ? | - |
? | |
pro-caspase-7 + H2O | activates caspase-7, whether of human or murine origin, with a similar efficiency | Mus musculus | ? | - |
? | |
pro-caspase-7 + H2O | activates caspase-7, whether of human or murine origin, with a similar efficiency | Homo sapiens | ? | - |
? |
Synonyms | Comment | Organism |
---|---|---|
GzmB | - |
Mus musculus |
GzmB | - |
Homo sapiens |