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Literature summary for 3.4.21.79 extracted from

  • Sun, J.; Bird, C.H.; Buzza, M.S.; McKee, K.E.; Whisstock, J.C.; Bird, P.I.
    Expression and purification of recombinant human granzyme B from Pichia pastoris (1999), Biochem. Biophys. Res. Commun., 261, 251-255.
    View publication on PubMed
Search for more literature for 3.4.21.79

Cloned(Commentary)

Cloned (Comment) Organism
recombinant granzyme B expressed in Pichia pastoris as a chimeric zymogen comprising the alpha-factor signal sequence, a prodomain including an enterokinase cleavage site, and the mature granzyme B sequence followed by a hexahistidine tag, cloned and expressed in Escherichia coli, resulting fusion protein is insoluble and folded incorrectly Homo sapiens

Inhibitors

Inhibitors Comment Organism Structure
serpin proteinase inhibitor 9
-
 Homo sapiens

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.082
-
 Boc-Ala-Ala-Asp-SBzl pH 7.5, 25°C, recombinant enzyme Homo sapiens
0.15
-
 Boc-Ala-Ala-Asp-SBzl pH 7.5, 25°C, native enzyme Homo sapiens

Organism

Organism UniProt Comment Textmining
Homo sapiens
-
 human
-

Purification (Commentary)

Purification (Comment) Organism
recombinant enzyme Homo sapiens

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
Boc-Ala-Ala-Asp-SBzl + H2O
-
 Homo sapiens ?
-
 ?
additional information induces DNA degradation and apoptosis of cells Homo sapiens ?
-
 ?

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
11
-
 Boc-Ala-Ala-Asp-SBzl pH 7.5, 25°C, native enzyme Homo sapiens
12
-
 Boc-Ala-Ala-Asp-SBzl pH 7.5, 25°C, recombinant enzyme Homo sapiens