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Literature summary for 3.4.21.79 extracted from

  • Xia, Z.; Kam, C.M.; Huang, C.; Powers, J.C.; Mandle, R.J.; Stevens, R.L.; Lieberman, J.
    Expression and purification of enzymatically active recombinant granzyme B in a baculovirus system (1998), Biochem. Biophys. Res. Commun., 243, 384-389.
    View publication on PubMed
Search for more literature for 3.4.21.79

Cloned(Commentary)

Cloned (Comment) Organism
recombinant granzyme B expressed from baculovirus in Sf9 cells Mus musculus

Inhibitors

Inhibitors Comment Organism Structure
Boc-Ile-Glu-Ala-Asp-CONH(CH2)2-Ph granB specific inhibitor Homo sapiens
Boc-Ile-Glu-Ala-Asp-CONH(CH2)2-Ph granB specific inhibitor Mus musculus

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.355
-
 Boc-Ala-Ala-Asp-SBzl pH 7.5, 23°C Mus musculus
0.683
-
 Boc-Ala-Ala-Asp-SBzl pH 7.5, 23°C> Homo sapiens

Localization

Localization Comment Organism GeneOntology No. Textmining
cytoplasm cytoplasmic granules of cytotoxic T lymphocytes and natural killer cells Mus musculus 5737
-
cytoplasm cytoplasmic granules of cytotoxic T lymphocytes and natural killer cells Homo sapiens 5737
-

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
27000
-
 predicted from cDNA Mus musculus
28000 33000 immunoblotting Mus musculus

Organism

Organism UniProt Comment Textmining
Homo sapiens
-
 human
-
Mus musculus
-
 mouse
-

Purification (Commentary)

Purification (Comment) Organism
-
 Homo sapiens
recombinant enzyme Mus musculus

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
Boc-Ala-Ala-Asp-SBzl + H2O
-
 Mus musculus ?
-
 ?
Boc-Ala-Ala-Asp-SBzl + H2O
-
 Homo sapiens ?
-
 ?
additional information requirement for aspartic acid in the substrate P1 position Homo sapiens ?
-
 ?
additional information requirement for aspartic acid in the substrate P1 position, induces DNA fragmentation in isolated nuclei in the presence of cytosolic factors Mus musculus ?
-
 ?
pro-CPP32 + H2O processes the caspase into its enzymatically active form Mus musculus ?
-
 ?

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
16
-
 Boc-Ala-Ala-Asp-SBzl pH 7.5, 23°C Mus musculus
18
-
 Boc-Ala-Ala-Asp-SBzl pH 7.5, 23°C Homo sapiens

Ki Value [mM]

Ki Value [mM] Ki Value maximum [mM] Inhibitor Comment Organism Structure
0.0084
-
 Boc-Ile-Glu-Ala-Asp-CONH(CH2)2-Ph pH 7.5, 23°C Homo sapiens
0.0123
-
 Boc-Ile-Glu-Ala-Asp-CONH(CH2)2-Ph pH 7.5, 23°C Mus musculus