Application | Comment | Organism |
---|---|---|
medicine | responsible for eliminating intracellular pathogens and breaking down tissues at inflammatory sites. Possible molecular target for anti-inflammatory agents. Involved in degradation of ECM components and cleavage of inflammatory mediators, platlet activation, induction of endothelial cells and apoptosis, negative feedback regulation of granulopoiesis. Shows bactericidal properties. Is a target of auto-antibodies in Wegeners granulomatosis | Homo sapiens |
Cloned (Comment) | Organism |
---|---|
expressed in insect cells | Homo sapiens |
Crystallization (Comment) | Organism |
---|---|
crystal structure of recombinant PR3. Overall fold consists of two beta-barrel domains. PR3 structure includes a disaccharide unit covalently attached to Asn159. PR3 substrate binding sites at S4 to S3' | Homo sapiens |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
alpha1-PI | - |
Homo sapiens | |
alpha2-Macroglobulin | - |
Homo sapiens | |
elafin | - |
Homo sapiens | |
MNEI | - |
Homo sapiens | |
additional information | transcription of PR3 is downregulated upon granulocyte and monocyte maturation | Homo sapiens | |
pre-elafin | - |
Homo sapiens |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
cell surface | granulocytes and monocytes | Homo sapiens | 9986 | - |
membrane | secretory vesicles and secondary granules | Homo sapiens | 16020 | - |
secondary granule | - |
Homo sapiens | - |
- |
secretory vesicle | - |
Homo sapiens | 99503 | - |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Homo sapiens | P24158 | - |
- |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
basophil | - |
Homo sapiens | - |
epithelial cell | inflamed oral epithelial cell | Homo sapiens | - |
granulocyte | active PR3 stored in neutrophil azurophil granules | Homo sapiens | - |
monocyte | - |
Homo sapiens | - |
additional information | mRNA for PR3 is transcribed in the promyelocyte and promonocyte stages of myeloid differentiation. Present in mastocytes and no-hematopoietic cell | Homo sapiens | - |
neutrophil | blood neutrophils from patients with active Wegeners granulomatosis bear greater amounts of PR3 on their cell membranes than do neutrophils from healthy individuals | Homo sapiens | - |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
Abz-VADCRDRQ-EDDnp + H2O | best synthetic substrate | Homo sapiens | Abz-VADCR + DRQ-EDDnp | - |
? | |
Elastin + H2O | - |
Homo sapiens | Hydrolyzed elastin | - |
? | |
FRET + H2O | - |
Homo sapiens | ? | - |
? | |
IL-18 + H2O | - |
Homo sapiens | ? | - |
? | |
IL-1beta + H2O | - |
Homo sapiens | ? | - |
? | |
additional information | substrate specificity for small hydrophobic residues at P1 position (Val, Cys, Ala, Met, Ser and Leu) | Homo sapiens | ? | - |
? | |
NF-kappaB + H2O | - |
Homo sapiens | ? | - |
? | |
oxidized insulin B chain + H2O | - |
Homo sapiens | ? | - |
? | |
p21 + H2O | - |
Homo sapiens | ? | - |
? | |
protease-activated receptor-2 + H2O | - |
Homo sapiens | ? | - |
? | |
TNF-alpha + H2O | - |
Homo sapiens | ? | - |
? |
Subunits | Comment | Organism |
---|---|---|
? | 29000-32000, SDS-PAGE | Homo sapiens |
Synonyms | Comment | Organism |
---|---|---|
PR3 | - |
Homo sapiens |
proteinase 3 | - |
Homo sapiens |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
8 | - |
- |
Homo sapiens |
pH Minimum | pH Maximum | Comment | Organism |
---|---|---|---|
additional information | - |
the mature PR3 has an inactive conformation in the acidic environment of the azurophilic granules, and becomes active upon translocation to a neutral pH environment | Homo sapiens |
Organism | Comment | pI Value Maximum | pI Value |
---|---|---|---|
Homo sapiens | - |
- |
9.5 |