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Literature summary for 3.4.21.75 extracted from
- Interaction of Mint3 with Furin regulates the localization of Furin in the trans-Golgi network (2008), J. Cell Sci., 121, 2217-2223.
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
| plasmid pCMV-furin expressed in HeLa cells. Catalytic domain, P domain and cytoplasmic domain of furin subcloned from full-length pCMV-furin and ligated in-frame to the C-terminus of GST using the PGEX-6P system | synthetic construct |
Protein Variants
| Protein Variants | Comment | Organism |
|---|---|---|
| DELTA759-780 | furin mutant lacking both the YKGL motif and the acidic cluster, shows low-affinity interactions with Mint3 | synthetic construct |
| DELTA769-780 | furin mutant with a deleted acidic cluster, shows low-affinity interactions with Mint3 | synthetic construct |
| additional information | the binding efficiency of the furin mutants LI/AN Y/A and LI/AN F/N Y/A to Mint3 decrease | synthetic construct |
Localization
| Localization | Comment | Organism | GeneOntology No. | Textmining |
|---|---|---|---|---|
| endosome | furin localizes to endosomes when Mint3 expression is knocked down | synthetic construct | 5768 | - |
| Golgi apparatus | interaction between the phosphotyrosine-binding domain of Mint3 and the acidic peptide signal in the cytoplasmic domain of furin regulates the specific localization of furin in the trans-Golgi network. The phosphotyrosine-binding domain of Mint3 is essential for furin-Mint3 interaction | synthetic construct | 5794 | - |
| membrane | knockdown of Mint3 increases the distribution of furin at the membrane | synthetic construct | 16020 | - |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 90000 | - |
immunoprecipitation, mature form of furin | synthetic construct |
| 96000 | - |
immunoprecipitation, proprotein furin | synthetic construct |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| synthetic construct | - |
- |
- |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| Proprotein convertase | - |
synthetic construct |
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Release 2023.1 (January 2023)
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