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Literature summary for 3.4.21.74 extracted from

  • Zaqueo, K.D.; Kayano, A.M.; Simoes-Silva, R.; Moreira-Dill, L.S.; Fernandes, C.F.; Fuly, A.L.; Maltarollo, V.G.; Honorio, K.M.; da Silva, S.L.; Acosta, G.; Caballol, M.A.; de Oliveira, E.; Albericio, F.; Calderon, L.A.; Soares, A.M.; Stabeli, R.G.
    Isolation and biochemical characterization of a new thrombin-like serine protease from Bothrops pirajai snake venom (2014), BioMed Res. Int., 2014, 595186.
    View publication on PubMedView publication on EuropePMC
Search for more literature for 3.4.21.74

Inhibitors

Inhibitors Comment Organism Structure
Cu2+
-
 Bothrops pirajai
PMSF
-
 Bothrops pirajai

Localization

Localization Comment Organism GeneOntology No. Textmining
extracellular
-
 Bothrops pirajai
-
-

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
39408
-
 x * 39408, mass spectrometry, x * 49000, SDS-PAGE Bothrops pirajai
49000
-
 x * 39408, mass spectrometry, x * 49000, SDS-PAGE Bothrops pirajai

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
factor XIII + H2O Bothrops pirajai activation of the factor in the blood coagulation cascade ?
-
 ?

Organism

Organism UniProt Comment Textmining
Bothrops pirajai
-
 from Brasil
-

Posttranslational Modification

Posttranslational Modification Comment Organism
glycoprotein molecular modeling and determination of N-glycosylation sites, overview Bothrops pirajai

Purification (Commentary)

Purification (Comment) Organism
native enzyme from venom by gel filtration, benzamidine affinity chromatography and reversed-phase gel filtration on a C2/C18 column Bothrops pirajai

Source Tissue

Source Tissue Comment Organism Textmining
venom
-
 Bothrops pirajai
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
D-Phe-Pip-Arg-4-nitroanilide + H2O chromogenic substrate Bothrops pirajai D-Phe-Pip-Arg + 4-nitroaniline
-
 ?
D-Pro-Phe-Arg-4-nitroanilide + H2O chromogenic substrate Bothrops pirajai D-Pro-Phe-Arg + 4-nitroaniline
-
 ?
factor XIII + H2O activation Bothrops pirajai ?
-
 ?
factor XIII + H2O activation of the factor in the blood coagulation cascade Bothrops pirajai ?
-
 ?
Gelatin + H2O degradation Bothrops pirajai ?
-
 ?
Nalpha-benzoyl-DL-arginyl 4-nitroanilide + H2O amidolytic activity Bothrops pirajai Nalpha-benzoyl-DL-arginine + 4-nitroaniline
-
 ?
S-2222 + H2O chromogenic substrate Bothrops pirajai ?
-
 ?

Subunits

Subunits Comment Organism
? x * 39408, mass spectrometry, x * 49000, SDS-PAGE Bothrops pirajai

Synonyms

Synonyms Comment Organism
BpirSP-39
-
 Bothrops pirajai
thrombin-like serine protease
-
 Bothrops pirajai

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
37
-
 assay at Bothrops pirajai

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7.4
-
 assay at Bothrops pirajai

General Information

General Information Comment Organism
additional information the catalytic triad comprises residues His42, Asp86, and Ser194, simulation of molecular dynamics, overview Bothrops pirajai
physiological function the enzyme is able to form fibrin networks. It promotes platelet aggregation and also exhibits fibrinogenolytic, fibrinolytic, gelatinolytic, and amidolytic activities Bothrops pirajai