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Literature summary for 3.4.21.74 extracted from
- Characterization of a novel pro-coagulant metalloprotease (RVBCMP) possessing alpha-fibrinogenase and tissue haemorrhagic activity from venom of Daboia russelli russelli (Russells viper): evidence of distinct coagulant and haemorrhagic sites in RVBCMP (2008), Toxicon, 51, 923-933.
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| dithiothreitol | at 2 mM completely inhibits coagulant and protease activities of RVBCMP | Daboia russelii russelii |  |
| EDTA | at 2 mM completely inhibits coagulant and protease activities of RVBCMP | Daboia russelii russelii | |
| additional information | treatment of RVBCMP with serine protease inhibitors phenylmethyl suphonyl fluoride and DIFP at concentrations ranging from 0.5 to 2 mM does not affect its haemorrhagic and protease activities, and at 2 mM concentration, only a minimal (2-4%) inhibition of coagulation activity occurs. Aqueous leaf extracts from Murraya koenigii, Alostonia scholaris and Azacardica indica, except Leucus lavandulaefolia, do not inhibit the coagulant or haemorrhagic activity of RVBCMP. Leucus lavandulaefolia leaf extract significantly inhibits the liver haemorrhagic activity but not the coagulant activity of RVBCMP | Daboia russelii russelii | |
| polyvalent antivenom | inhibits the coagulant, protease, and haemorrhagic activities of RVBCMP in a dose-dependent manner, but to a different extent | Daboia russelii russelii |
Metals/Ions
| Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|
| Ca2+ | has the highest stimulating effect of all metal ion at 2 mM, maximum coagulant activity in the presence of 7 mM Ca2+ | Daboia russelii russelii | |
| Cu2+ | at 2 mM 10% less active than Ca2+ | Daboia russelii russelii | |
| Mg2+ | at 2 mM 19% less active than Ca2+ | Daboia russelii russelii | |
| Mn2+ | has the least influence on RVBCMP-induced coagulation of human plasma, at 2 mM 30% less active than Ca2+ | Daboia russelii russelii | |
| Ni2+ | at 2 mM 12% less active than Ca2+ | Daboia russelii russelii | |
| Zn2+ | at 2 mM 8% less active than Ca2+ | Daboia russelii russelii | |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 15000 | - |
1 * 15000, SDS-PAGE, after reduction with beta-mercaptoethanol. 1 * 21000, SDS-PAGE, in the absence of reducing agent and heating | Daboia russelii russelii |
| 15090 | - |
MALDI-TOF-mass spectrometry | Daboia russelii russelii |
| 21000 | - |
1 * 15000, SDS-PAGE, after reduction with beta-mercaptoethanol. 1 * 21000, SDS-PAGE, in the absence of reducing agent and heating | Daboia russelii russelii |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Daboia russelii russelii | - |
- |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| by gel filtration, 3fold | Daboia russelii russelii |
Source Tissue
| Source Tissue | Comment | Organism | Textmining |
|---|---|---|---|
| venom | - |
Daboia russelii russelii | - |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| 13 | - |
crude venom, equivalent of tyrosine formed per minute | Daboia russelii russelii |
| 49 | - |
3fold purified enzyme, equivalent of tyrosine formed per minute | Daboia russelii russelii |
Storage Stability
| Storage Stability | Organism |
|---|---|
| 4°C, 7 days | Daboia russelii russelii |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| additional information | RVBCMP exerts dose-dependent coagulation of platelet-poor human plasma. RVBCMP does not show oedema induction, direct haemolysis of washed erythrocytes, hydrolysis of human plasma albumin or globulin, and thrombin-like activity, but exhibits caseinolytic, alpha-fibrinogenolytic, and liver tissue haemorrhagic activities. RVBCMP up to a dose of 25 mg/ml has no detectable phospholipase A2, acetylcholinesterase, and ATPase activities | Daboia russelii russelii | ? | - |
? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| monomer | 1 * 15000, SDS-PAGE, after reduction with beta-mercaptoethanol. 1 * 21000, SDS-PAGE, in the absence of reducing agent and heating | Daboia russelii russelii |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| Russell's viper basic coagulant metalloprotease | - |
Daboia russelii russelii |
| RVBCMP | - |
Daboia russelii russelii |
Temperature Stability [°C]
| Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 40 | - |
heating the RVBCMP at 40°C for 60 min at pH 7.4 does not influence the coagulant activity of the protein, whereas heating RVBCMP at 55°C for 20 min over a pH range from 7.0 to 8.0 results in only 42% of the original clotting activity remaining. Heating the RVBCP at 60°C results in a complete loss of coagulant activity | Daboia russelii russelii |
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