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Literature summary for 3.4.21.74 extracted from
- Expression and characterization of a recombinant fibrinogenolytic serine protease from green pit viper (Trimeresurus albolabris) venom (2007), Toxicon, 49, 1083-1089.
Application
| Application | Comment | Organism |
|---|---|---|
| medicine | potentially useful as antithrombotic defibrinating agent. Albofibrase effects can be rapidly reversed using commercial available antivenom. The enzyme shows anticoagulant activity, it progressively prolongs prothrombin time and activated partial thromboplastin time of normal human plasma in a time-dependent manner | Trimeresurus albolabris |
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
| recombinant albofibrase1 expressed in Pichia pastoris strain X-33 | Trimeresurus albolabris |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 27800 | - |
sequence analysis | Trimeresurus albolabris |
| 30000 | - |
x * 30000, SDS-PAGE, recombinant active enzyme including 2.2 kDa of glycosylation | Trimeresurus albolabris |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Trimeresurus albolabris | - |
- |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| by gel filtration | Trimeresurus albolabris |
Source Tissue
| Source Tissue | Comment | Organism | Textmining |
|---|---|---|---|
| venom | - |
Trimeresurus albolabris | - |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| Boc-Leu-Ser-Thr-Arg-p-nitroanilide + H2O | weaker proteolytic activity than with Bz-DL-Arg-p-nitroaniline | Trimeresurus albolabris | p-nitroaniline + Boc-Leu-Ser-Thr-Arg | - |
? |  |
| Bz-DL-Arg-p-nitroanilide + H2O | proteolytic activity | Trimeresurus albolabris | p-nitroaniline + Bz-DL-Arg | - |
? | |
| Fibrinogen + H2O | cleaves peptide bonds in the Aalpha-chain | Trimeresurus albolabris | ? | - |
? | |
| additional information | no activity with Bz-Tyr-p-nitroaniline | Trimeresurus albolabris | ? | - |
? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| ? | x * 30000, SDS-PAGE, recombinant active enzyme including 2.2 kDa of glycosylation | Trimeresurus albolabris |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| albofibrase1 | - |
Trimeresurus albolabris |
| alpha fibrinogenase | - |
Trimeresurus albolabris |
| viper venom serine protease | - |
Trimeresurus albolabris |
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Release 2023.1 (January 2023)
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