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Literature summary for 3.4.21.74 extracted from
- Isolation of multiple isoforms of alpha-fibrinogenase from the Western diamondback rattlesnake, Crotalus atrox: N-terminal sequence homology with ancrod, an antithrombotic agent from Malayan viper (1994), Biochem. Biophys. Res. Commun., 201, 1414-1423.
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| Aprotinin | from bovine | Crotalus atrox |  |
| additional information | no inhibition by EDTA, 2-mercaptoethanol | Crotalus atrox | |
| PMSF | - |
Crotalus atrox | |
| Soybean trypsin inhibitor | - |
Crotalus atrox |
Localization
| Localization | Comment | Organism | GeneOntology No. | Textmining |
|---|---|---|---|---|
| soluble | - |
Crotalus atrox | - |
- |
Metals/Ions
| Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|
| additional information | no metalloproteinase | Crotalus atrox | |
| additional information | no activation by Ca2+ | Crotalus atrox |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| additional information | - |
N-terminal sequence | Crotalus atrox |
| 30000 | 35000 | Crotalus atrox, gel filtration | Crotalus atrox |
| 33000 | - |
1 * 33000, Crotalus atrox, isozymes A1 and A3, SDS-PAGE | Crotalus atrox |
| 35000 | - |
1 * 35000, Crotalus atrox, isozyme A2, SDS-PAGE | Crotalus atrox |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| Fibrinogen + H2O | Crotalus atrox | three of numerous fibrinogenases of Crotalus atrox venom | ? | - |
? | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Crotalus atrox | - |
Western diamondback rattlesnake | - |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| multiple isoforms | Crotalus atrox |
Source Tissue
| Source Tissue | Comment | Organism | Textmining |
|---|---|---|---|
| venom | - |
Crotalus atrox | - |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| Fibrinogen + H2O | three of numerous fibrinogenases of Crotalus atrox venom | Crotalus atrox | ? | - |
? | |
| Fibrinogen Aalpha-chains + H2O | - |
Crotalus atrox | ? | - |
? | |
| fibrinogen B-beta-chain + H2O | - |
Crotalus atrox | ? | - |
? | |
| additional information | fibrinogen gamma-chain | Crotalus atrox | ? | - |
? | |
| Oxidized insulin B-chain + H2O | cleavage sites: Tyr16-Leu17 (isozymes A1-3), Gly23-Phe24, Phe25-Val26 (isozymes A1 and 2), His5-Leu6 (isozyme A1), His10-Leu11, Leu15-Tyr16, Phe24-Phe25 (isozyme A2) | Crotalus atrox | Hydrolyzed insulin B-chain | - |
? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| monomer | 1 * 33000, Crotalus atrox, isozymes A1 and A3, SDS-PAGE | Crotalus atrox |
| monomer | 1 * 35000, Crotalus atrox, isozyme A2, SDS-PAGE | Crotalus atrox |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 37 | - |
assay at | Crotalus atrox |
Temperature Stability [°C]
| Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 75 | - |
30 min, stable, isozyme A3 | Crotalus atrox |
| 85 | - |
30 min, stable, isozymes A1 and 2 | Crotalus atrox |
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