Literature summary for 3.4.21.74 extracted from

Simmons, G.; Bundalian, M.; Theodor, I.; Martinoli, J.; Pirkle, H.
Action of crotalase, an enzyme with thrombin-like and kallikrein-like specificities, on tripeptide nitroanilide derivatives (1985), Thromb. Res., 40, 555-561.

Search for more literature for 3.4.21.74

Inhibitors

Inhibitors	Comment	Organism	Structure
H-D-Nle-cyclohexylalanyl-Arg 4-nitroanilide	substrate inhibition, 1.24 mM and above	Crotalus adamanteus
H-D-Phenylglycyl-Phe-Arg 4-nitroanilide	substrate inhibition, 0.31 mM and above	Crotalus adamanteus

KM Value [mM]

KM Value [mM]	KM Value Maximum [mM]	Substrate	Comment	Organism	Structure
0.025	-	carbobenzoxyl-Glu-(alpha-tert-butoxy)-Gly-Arg-4-nitroanilide	-	Crotalus adamanteus
0.058	-	H-D-Pro-hexahydrotyrosyl-Arg 4-nitroanilide	-	Crotalus adamanteus
0.18	-	D-Phe-L-pipecoyl-L-Arg 4-nitroanilide	H-D-cyclohexylglycyl-L-alpha-aminobutyryl-Arg 4-nitroanilide	Crotalus adamanteus
0.27	-	H-D-Nle-cyclohexylalanyl-Arg 4-nitroanilide	H-D-phenylglycyl-Phe-Arg 4-nitroanilide	Crotalus adamanteus
0.3	-	Tosyl-Gly-Pro-Arg 4-nitroanilide	-	Crotalus adamanteus
0.39	-	benzoyl-Pro-Phe-Arg 4-nitroanilide	-	Crotalus adamanteus
0.45	-	H-D-Hexahydrotyrosyl-Ala-Arg 4-nitroanilide	-	Crotalus adamanteus
1.4	-	tosyl-Gly-Pro-Lys 4-nitroanilide	-	Crotalus adamanteus
2.7	-	H-D-Nle-hexahydrotyrosyl-Lys 4-nitroanilide	-	Crotalus adamanteus
3.3	-	H-D-Aminobutyric acid-cyclohexylalanyl-Lys 4-nitroanilide	methylsulfonyl-Leu-Gly-Arg 4-nitroanilide	Crotalus adamanteus

Organism

Organism	UniProt	Comment	Textmining
Crotalus adamanteus	-	Eastern diamondback rattlesnake, formerly EC 3.4.21.30	-

Source Tissue

Source Tissue	Comment	Organism	Textmining
venom	-	Crotalus adamanteus	-

Substrates and Products (Substrate)

Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.	Reac.
Benzoyl-Pro-Phe-Arg 4-nitroanilide + H2O	-	Crotalus adamanteus	Benzoyl-Pro-Phe-Arg + 4-nitroaniline	-	?
benzyloxycarbonyl-Phe 4-nitrophenyl ester + H2O	-	Crotalus adamanteus	benzyloxycarbonyl-Phe + 4-nitrophenol	-	?
Carbobenzoxyl-Glu-(alpha-t-butoxy)-Gly-Arg 4-nitroanilide + H2O	-	Crotalus adamanteus	Carbobenzoxyl-Glu-(alpha-t-butoxy)-Gly-Arg + 4-nitroaniline	-	?
carbobenzoxyl-Glu-(alpha-tert-butoxy)-Gly-Arg-4-nitroanilide + H2O	-	Crotalus adamanteus	carbobenzoxyl-Glu-(alpha-tert-butoxy)-Gly-Arg + 4-nitroaniline	-	?
D-aminobutyric acid-cyclohexylalanyl-Lys 4-nitroanilide + H2O	-	Crotalus adamanteus	D-aminobutyric acid-cyclohexylalanyl-Lys + 4-nitroaniline	-	?
D-Phe-L-pipecoyl-L-Arg 4-nitroanilide + H2O	-	Crotalus adamanteus	?	-	?
H-D-Cyclohexylglycyl-aminobutyric acid-cyclohexylalanyl-Lys 4-nitroanilide + H2O	-	Crotalus adamanteus	H-D-Cyclohexylglycyl-aminobutyric acid-cyclohexylalanyl-Lys + 4-nitroaniline	-	?
H-D-Cyclohexylglycyl-L-2-aminobutyryl-Arg 4-nitroanilide + H2O	-	Crotalus adamanteus	H-D-Cyclohexylglycyl-L-alpha-aminobutyryl-L-arginine + 4-nitroaniline	-	?
H-D-Hexahydrotyrosyl-Ala-Arg 4-nitroanilide + H2O	-	Crotalus adamanteus	H-D-Hexahydrotyrosyl-Ala-Arg + 4-nitroaniline	-	?
H-D-Nle-cyclohexylalanyl-Arg 4-nitroanilide + H2O	-	Crotalus adamanteus	H-D-Nle-cyclohexylalanyl-L-arginine + 4-nitroaniline	-	?
H-D-Nle-hexahydrotyrosyl-Lys 4-nitroanilide + H2O	-	Crotalus adamanteus	H-D-Nle-hexahydrotyrosyl-Lys + 4-nitroaniline	-	?
H-D-Phenylglycyl-Phe-Arg 4-nitroanilide + H2O	-	Crotalus adamanteus	H-D-Phenylglycyl-Phe-Arg + 4-nitroaniline	-	?
H-D-Pro-hexahydrotyrosyl-Arg 4-nitroanilide + H2O	-	Crotalus adamanteus	H-D-Pro-hexahydrotyrosyl-L-arginine + 4-nitroaniline	-	?
Methylsulfonyl-Leu-Gly-Arg 4-nitroanilide + H2O	-	Crotalus adamanteus	Methylsulfonyl-Leu-Gly-Arg + 4-nitroaniline	-	?
additional information	no plasmin-like activity	Crotalus adamanteus	?	-	?
additional information	specificity compared to that of thrombin and kallikrein	Crotalus adamanteus	?	-	?
additional information	hydrolyzes Arg-bonds better than Lys-bonds	Crotalus adamanteus	?	-	?
Tosyl-Gly-Pro-Arg 4-nitroanilide + H2O	-	Crotalus adamanteus	Tosyl-Gly-Pro-Arg + 4-nitroaniline	-	?
Tosyl-Gly-Pro-Lys 4-nitroanilide + H2O	-	Crotalus adamanteus	Tosyl-Gly-Pro-Lys + 4-nitroaniline	-	?

Temperature Optimum [°C]

Temperature Optimum [°C]	Temperature Optimum Maximum [°C]	Comment	Organism
27	-	assay at	Crotalus adamanteus

Turnover Number [1/s]

Turnover Number Minimum [1/s]	Turnover Number Maximum [1/s]	Substrate	Comment	Organism	Structure
0.16	-	carbobenzoxyl-Glu-(alpha-tert-butoxy)-Gly-Arg-4-nitroanilide	-	Crotalus adamanteus
0.35	-	benzoyl-Pro-Phe-Arg 4-nitroanilide	-	Crotalus adamanteus
0.68	-	H-D-Cyclohexylglycyl-L-2-aminobutyryl-Arg 4-nitroanilide	-	Crotalus adamanteus
0.7	-	tosyl-Gly-Pro-Lys 4-nitroanilide	-	Crotalus adamanteus
0.95	-	H-D-Hexahydrotyrosyl-Ala-Arg 4-nitroanilide	-	Crotalus adamanteus
1.3	-	Methylsulfonyl-Leu-Gly-Arg 4-nitroanilide	-	Crotalus adamanteus
1.4	-	H-D-Aminobutyric acid-cyclohexylalanyl-Lys 4-nitroanilide	-	Crotalus adamanteus
3.5	-	D-phenylglycyl-Phe-Arg 4-nitroanilide	-	Crotalus adamanteus
4.9	-	H-D-Pro-hexahydrotyrosyl-Arg 4-nitroanilide	-	Crotalus adamanteus
6.9	-	H-D-Nle-hexahydrotyrosyl-Lys 4-nitroanilide	-	Crotalus adamanteus
11.5	-	Tosyl-Gly-Pro-Arg 4-nitroanilide	-	Crotalus adamanteus
61	-	H-D-Nle-cyclohexylalanyl-Arg 4-nitroanilide	-	Crotalus adamanteus

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Release 2023.1 (January 2023)