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Literature summary for 3.4.21.73 extracted from

  • Makarova, A.M.; Lebedeva, T.V.; Nassar, T.; Higazi, A.A.; Xue, J.; Carinato, M.E.; Bdeir, K.; Cines, D.B.; Stepanova, V.
    Urokinase-type plasminogen activator (uPA) induces pulmonary microvascular endothelial permeability through low density lipoprotein receptor-related protein (LRP)-dependent activation of endothelial nitric-oxide synthase (2011), J. Biol. Chem., 286, 23044-23053.
    View publication on PubMedView publication on EuropePMC

Protein Variants

Protein Variants Comment Organism
S356A a catalytically inactive uPA mutant Homo sapiens

Inhibitors

Inhibitors Comment Organism Structure
additional information no inhibition by LY294002 and AktI Homo sapiens
myristoylated PKI mPLI, a protein kinase A inhibitor, complete inhibition Homo sapiens
PA inhibitor type 1 PAI-1, effects of uPA-PAI-1 are abrogated by the nitric-oxide synthase inhibitor N-D-nitro-L-arginine methyl ester. Dramatically elevated levels in case of acute lung injury Homo sapiens

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
additional information Homo sapiens uPA-induced phosphorylation of endothelial nitric oxide synthase, eNOS, which is inhibited by myristoylated PKI, a protein kinase A inhibitor ?
-
?

Organism

Organism UniProt Comment Textmining
Homo sapiens
-
-
-

Source Tissue

Source Tissue Comment Organism Textmining
lung
-
Homo sapiens
-
pulmonary microvascular endothelial cell
-
Homo sapiens
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
additional information uPA-induced phosphorylation of endothelial nitric oxide synthase, eNOS, which is inhibited by myristoylated PKI, a protein kinase A inhibitor Homo sapiens ?
-
?

Synonyms

Synonyms Comment Organism
uPA
-
Homo sapiens
Urokinase-type plasminogen activator
-
Homo sapiens

General Information

General Information Comment Organism
physiological function two-chain active uPA and uPA-PAI-1 enzyme-inhibitor complex induce phosphorylation of endothelial NOS-Ser1177 in pulmonary microvascular endothelial cells, which is followed by generation of NO and the nitrosylation and dissociation of beta-catenin from VE-cadherin, mechanism of uPA-induced pulmonary vascular permeability in vivo, overview. Effects of uPA-PAI-1 are abrogated by the nitric-oxide synthase inhibitor N-D-nitro-L-arginine methyl ester. The PI3K/Akt pathway is not essential for uPA-induced phosphorylation of eNOS Homo sapiens