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Literature summary for 3.4.21.73 extracted from

  • Barinka, C.; Parry, G.; Callahan, J.; Shaw, D.E.; Kuo, A.; Bdeir, K.; Cines, D.B.; Mazar, A.; Lubkowski, J.
    Structural basis of interaction between urokinase-type plasminogen activator and its receptor (2006), J. Mol. Biol., 363, 482-495.
    View publication on PubMedView publication on EuropePMC

Cloned(Commentary)

Cloned (Comment) Organism
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Homo sapiens

Crystallization (Commentary)

Crystallization (Comment) Organism
purified amino-terminal fragment of urokinase-type plasminogen activator, free and complexed with uPA receptor, lacking the cell-surface anchoring sequence, hanging-drop vapor-diffusion method, room temperature, 0.003 ml of 20 or 8 mg/ml protein, respectively, in 20 mM Tris-HCl, pH 8.0, 150 mM NaCl, is mixed with an equal volume of reservoir solution containing 100 mM Ches, pH 10.5, 1.2 M sodium dihydrogen phosphate, 0.8 M potassium hydrogen phosphate, 200 mM lithium sulfate for the free enzyme or 100 mM Bis-Tris, pH 5.5, 22.5% w/v PEG 3350, 200 mM ammonium sulfate for the enzyme fragment-receptor complex, 1 week, cryoprotection by 15% v/v glycerol, X-ray diffraction structure determination and anaylsis at 1.9-3.3 A resolution, molecular modeling Homo sapiens

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
additional information Homo sapiens structure and interdomain contacts of the N-terminal domain of the enzyme with the uPA receptor, binding structure, mechanisms responsible for the cellular responses induced by uPA binding, overview ?
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?

Organism

Organism UniProt Comment Textmining
Homo sapiens
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-
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Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
additional information structure and interdomain contacts of the N-terminal domain of the enzyme with the uPA receptor, binding structure, mechanisms responsible for the cellular responses induced by uPA binding, overview Homo sapiens ?
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?

Synonyms

Synonyms Comment Organism
uPA
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Homo sapiens
Urokinase-type plasminogen activator
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Homo sapiens