Any feedback?
Literature summary for 3.4.21.72 extracted from
- The expression of soluble and active recombinant Haemophilus influenzae IgA1 protease in E. coli (2010), J. Biomed. Biotechnol., 2010, 253983.
Application
| Application | Comment | Organism |
|---|---|---|
| medicine | the IgA1 proteases may have the potential to cleave IgA1 complexes in the human host kidney and be a therapeutic agent for IgA1 nephropathy, a disease characterized by deposition of the IgA1 antibody in the glomerulus | Haemophilus influenzae |
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
| expression of His-tagged IgA1 protease in Escherichia coli strain C41(DE3), method development and optimization by evaluation of expression of recombinant Haemophilus influenzae IgA1 protease by combining various expression plasmids, IgA1 protease constructs, and Escherichia coli strains under multiple conditions, overview | Haemophilus influenzae |
Localization
| Localization | Comment | Organism | GeneOntology No. | Textmining |
|---|---|---|---|---|
| extracellular | - |
Haemophilus influenzae | - |
- |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| human IgA1 + H2O | Haemophilus influenzae | the enzyme specifically cleaves the hinge region of human IgA1, the predominant class of immunoglobulin present on mucosal membranes | ? | - |
? |  |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Haemophilus influenzae | P44969 | - |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| recombinant His-tagged IgA1 protease by nickel affinity chromatography, anion exchange chromatography, and gel filtration | Haemophilus influenzae |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| human IgA1 + H2O | - |
Haemophilus influenzae | ? | - |
? | |
| human IgA1 + H2O | the enzyme specifically cleaves the hinge region of human IgA1, the predominant class of immunoglobulin present on mucosal membranes | Haemophilus influenzae | ? | - |
? | |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| IgA1 protease | - |
Haemophilus influenzae |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 37 | - |
assay at | Haemophilus influenzae |
Use of this online version of BRENDA is free under the CC BY 4.0 license. See terms of use for full details.
Release 2023.1 (January 2023)
Legal
Curated at
Member of
