Cloned (Comment) | Organism |
---|---|
gene iga, DNA and amino acid sequence determination and analysis of NMB IgA1 protease, sequence comparisons, expression of wild-type an dmutant enzymes in Escherichia coli strain XL 1-Blue | Neisseria meningitidis |
Protein Variants | Comment | Organism |
---|---|---|
additional information | construction of deletion or point mutants lacking the previously defined consensus cleavage site, the mutants are still able for self-cleavage and subsequent extracellular release of mature IgA1 protease | Neisseria meningitidis |
P1004A/S1005T/P1006A | site-directed mutagenesis, mutation of the cleavage recognition sequence residues | Neisseria meningitidis |
P1006X | site-directed mutagenesis, mutation of a cleavage recognition sequence residue, the mutant is still able for self-cleavage and subsequent extracellular release of mature IgA1 protease | Neisseria meningitidis |
S1005E | site-directed mutagenesis, mutation of a cleavage recognition sequence residue, the mutant is still able for self-cleavage and subsequent extracellular release of mature IgA1 protease | Neisseria meningitidis |
S267V | site-directed mutagenesis, mutation of the active site catalytic residue, inactive mutant, no self-cleavage and thus no secretion of the enzyme | Neisseria meningitidis |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
extracellular | active mature enzyme, the cleaved and maturated protein is secreted | Neisseria meningitidis | - |
- |
intracellular | inactive pro-enzyme, the cleaved and maturated protein is secreted | Neisseria meningitidis | 5622 | - |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
human immunoglobulin A1 + H2O | Neisseria meningitidis | bacterial isolates show wide variability in IgA1 protease activity, and those isolated from patients with clinical infection possess the highest levels of activity, overview | ? | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Neisseria meningitidis | Q51169 | iga; gene iga | - |
Posttranslational Modification | Comment | Organism |
---|---|---|
proteolytic modification | self-cleavage is required for secretion of the mature extracellular enzyme form, cleavage targets contain a proline-rich consensus recognition sequence, Pro-Pro-Ser-Pro, residing in the variable linker region that connects the protease and translocator domains, the precise amino acid sequence of the intervening region, between mature IgA1 protease and the beta-core translocator domain, influences the efficiency of autoproteolytic processing | Neisseria meningitidis |
Reaction | Comment | Organism | Reaction ID |
---|---|---|---|
Cleavage of immunoglobulin A molecules at certain Pro-/- bonds in the hinge region. No small molecule substrates are known | the catalytic serine residue is Ser267 | Neisseria meningitidis |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
additional information | bacterial isolates show wide variability in IgA1 protease activity, and those isolated from patients with clinical infection possess the highest levels of activity, overview | Neisseria meningitidis | - |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
human immunoglobulin A1 + H2O | bacterial isolates show wide variability in IgA1 protease activity, and those isolated from patients with clinical infection possess the highest levels of activity, overview | Neisseria meningitidis | ? | - |
? | |
human immunoglobulin A1 + H2O | site-specific proteolytic cleavage | Neisseria meningitidis | ? | - |
? | |
additional information | the NMB IgA1 protease shows a broad substrate specificity for human proteins | Neisseria meningitidis | ? | - |
? |
Subunits | Comment | Organism |
---|---|---|
More | secondary-structure analysis | Neisseria meningitidis |
Synonyms | Comment | Organism |
---|---|---|
IgA1 protease | - |
Neisseria meningitidis |
immunoglobulin A1 protease | - |
Neisseria meningitidis |
NMB IgA1 protease | - |
Neisseria meningitidis |
serine-type IgA1 protease | - |
Neisseria meningitidis |
serine-type immunoglobulin A1 protease | - |
Neisseria meningitidis |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
37 | - |
assay at | Neisseria meningitidis |