Cloned (Comment) | Organism |
---|---|
cDNA clone isolated | Rattus norvegicus |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
3,4-dichloroisocoumarin | causes total inhibition | Rattus norvegicus | |
diisopropyl fluorophosphate | causes total inhibition | Rattus norvegicus | |
additional information | enzyme activity is not affected by TPCK nor by phosphoramidon | Rattus norvegicus | |
N-alpha-tosyl-L-lysine-chloromethyl ketone | most potent inhibitor | Rattus norvegicus | |
phenylmethylsulfonyl fluoride | 1 mM, 50% inhibition | Rattus norvegicus | |
Soybean trypsin inhibitor | most potent inhibitor | Rattus norvegicus |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.089 | - |
Somatostatin-14 | pH 8.0, 25°C | Rattus norvegicus | |
1.567 | - |
Suc-Ala-Ala-Pro-Met-p-nitroanilide | pH 8.0, 25°C | Rattus norvegicus |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
additional information | increasing Ca2+ activity has no effect on elastase activity | Rattus norvegicus |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
27260 | - |
predicted from cDNA clone | Rattus norvegicus |
29000 | - |
SDS-PAGE | Rattus norvegicus |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
Somatostatin-14 + H2O | Rattus norvegicus | hydrolytic enzyme required for intestinal digestion of food stuff, natural substrates are proteins, protein fragments and peptides, greater affinity for natural substrates than for synthetics, specifically involved in the regulation of biological functions of somatostatin-14 in the gastrointestinal tract | ? | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Rattus norvegicus | - |
rat, male Wistar | - |
Posttranslational Modification | Comment | Organism |
---|---|---|
additional information | no carbohydrate moiety detected in the structure | Rattus norvegicus |
Purification (Comment) | Organism |
---|---|
- |
Rattus norvegicus |
Source Tissue | Comment | Organism | Textmining |
---|
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
additional information | - |
specific activity 118.83, activity of the enzyme is determined by its ability to degrade 125I-S-14, ng degraded/mg protein | Rattus norvegicus |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
additional information | elastin-Congo red is no substrate | Rattus norvegicus | ? | - |
? | |
SMS 201-995 + H2O | - |
Rattus norvegicus | ? | - |
? | |
Somatostatin-14 + H2O | - |
Rattus norvegicus | ? | - |
? | |
Somatostatin-14 + H2O | hydrolytic enzyme required for intestinal digestion of food stuff, natural substrates are proteins, protein fragments and peptides, greater affinity for natural substrates than for synthetics, specifically involved in the regulation of biological functions of somatostatin-14 in the gastrointestinal tract | Rattus norvegicus | ? | - |
? | |
Suc-Ala-Ala-Pro-Met-p-nitroanilide + H2O | - |
Rattus norvegicus | p-nitroaniline + Suc-Ala-Ala-Pro-Met | - |
? | |
Suc-Ala-Ala-Val-Ala-p-nitroanilide + H2O | - |
Rattus norvegicus | p-nitroaniline + Suc-Ala-Ala-Val-Ala | - |
? | |
Suc-Tyr-Leu-Val-p-nitroanilide + H2O | - |
Rattus norvegicus | p-nitroaniline + Suc-Tyr-Leu-Val | - |
? |
Subunits | Comment | Organism |
---|---|---|
monomer | 1 * 29000, SDS-PAGE | Rattus norvegicus |
pH Minimum | pH Maximum | Comment | Organism |
---|---|---|---|
5 | 9 | - |
Rattus norvegicus |
Organism | Comment | pI Value Maximum | pI Value |
---|---|---|---|
Rattus norvegicus | isoelectric focusing | 5.9 | 5.6 |