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Literature summary for 3.4.21.69 extracted from

  • Satou, M.; Nishi, Y.; Hishinuma, A.; Hosoda, H.; Kangawa, K.; Sugimoto, H.
    Identification of activated protein C as a ghrelin endopeptidase in bovine plasma (2015), J. Endocrinol., 224, 61-73.
    View publication on PubMed

Activating Compound

Activating Compound Comment Organism Structure
ProTac a protein C-activating agent Homo sapiens
ProTac a protein C-activating agent Bos taurus
ProTac a protein C-activating agent isolated from snake venom, administration to mice enhances the production of octanoyl ghrelin(1-15) in circulation Mus musculus

Inhibitors

Inhibitors Comment Organism Structure
ProTac a protein C activator isolated from snake venom Bos taurus

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
ghrelin + H2O Mus musculus ghrelin is converted into smaller fragments in blood plasma in circulation under thrombotic and inflammatory conditions ?
-
?
ghrelin + H2O Homo sapiens ghrelin is converted into smaller fragments in blood plasma in circulation under thrombotic and inflammatory conditions ?
-
?
ghrelin + H2O Bos taurus ghrelin is converted into smaller fragments in blood plasma in circulation under thrombotic and inflammatory conditions ?
-
?
ghrelin + H2O Mus musculus C57/BL6J ghrelin is converted into smaller fragments in blood plasma in circulation under thrombotic and inflammatory conditions ?
-
?

Organism

Organism UniProt Comment Textmining
Bos taurus
-
-
-
Homo sapiens
-
-
-
Mus musculus
-
-
-
Mus musculus C57/BL6J
-
-
-

Purification (Commentary)

Purification (Comment) Organism
native enzyme from bovine plasma by gel filtration, mannose affinity and Blue Sepharose chromatography Bos taurus

Source Tissue

Source Tissue Comment Organism Textmining
blood plasma
-
Mus musculus
-
blood plasma
-
Homo sapiens
-
blood plasma
-
Bos taurus
-
commercial preparation from plasma Bos taurus
-

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
57.3
-
purified native enzyme, amidolytic activity, pH 8.0, 37°C Bos taurus

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
Boc-Leu-Ser-Thr-Arg-4-nitroanilide + H2O
-
Mus musculus Boc-Leu-Ser-Thr-Arg + 4-nitroaniline
-
?
Boc-Leu-Ser-Thr-Arg-4-nitroanilide + H2O amidolytic activity Bos taurus Boc-Leu-Ser-Thr-Arg + 4-nitroaniline
-
?
Boc-Leu-Ser-Thr-Arg-4-nitroanilide + H2O
-
Mus musculus C57/BL6J Boc-Leu-Ser-Thr-Arg + 4-nitroaniline
-
?
ghrelin + H2O ghrelin is converted into smaller fragments in blood plasma in circulation under thrombotic and inflammatory conditions Mus musculus ?
-
?
ghrelin + H2O ghrelin is converted into smaller fragments in blood plasma in circulation under thrombotic and inflammatory conditions Homo sapiens ?
-
?
ghrelin + H2O ghrelin is converted into smaller fragments in blood plasma in circulation under thrombotic and inflammatory conditions Bos taurus ?
-
?
ghrelin + H2O ghrelin is converted into smaller fragments in blood plasma in circulation under thrombotic and inflammatory conditions Mus musculus C57/BL6J ?
-
?
additional information both acyl and desacyl ghrelin are hydrolyzed at the peptide bond between Arg15 and Lys16, generating an N-terminal peptide consisting of the first 15 residues Mus musculus ?
-
?
additional information both acyl and desacyl ghrelin are hydrolyzed at the peptide bond between Arg15 and Lys16, generating an N-terminal peptide consisting of the first 15 residues Homo sapiens ?
-
?
additional information bovine enzyme preferentially hydrolyzes the major form of octanoylated ghrelin(28), i.e. about twice as much hydrolysis as is observed for a longer acyl chain of decanoylated ghrelin(28). But both acyl and desacyl ghrelin are hydrolyzed at the peptidebond between Arg15 and Lys16, generating an N-terminal peptide consisting of the first 15 residues Bos taurus ?
-
?
additional information both acyl and desacyl ghrelin are hydrolyzed at the peptide bond between Arg15 and Lys16, generating an N-terminal peptide consisting of the first 15 residues Mus musculus C57/BL6J ?
-
?
octanoyl-ghrelin + H2O
-
Homo sapiens ?
-
?
octanoyl-ghrelin + H2O
-
Bos taurus ?
-
?
octanoyl-ghrelin + H2O preferred substrate Mus musculus octanoyl ghrelin(1-15) + ?
-
?
octanoyl-ghrelin + H2O preferred substrate Mus musculus C57/BL6J octanoyl ghrelin(1-15) + ?
-
?
octanoyl-truncated ghrelin + H2O preferred substrate, synthetic human substrate, octanoyl-truncated ghrelin(1-15) activates GHSR1a-dependent signaling similar to the full-length peptide Homo sapiens ?
-
?
octanoyl-truncated ghrelin + H2O preferred substrate, synthetic human substrate, octanoyl-truncated ghrelin(1-15) activates GHSR1a-dependent signaling similar to the full-length peptide Bos taurus ?
-
?
octanoyl-truncated ghrelin + H2O octanoyl-truncated ghrelin(1-15) activates GHSR1a-dependent signaling similar to the full-length peptide Mus musculus ghrelin(1-15) + ?
-
?
octanoyl-truncated ghrelin + H2O octanoyl-truncated ghrelin(1-15) activates GHSR1a-dependent signaling similar to the full-length peptide Mus musculus C57/BL6J ghrelin(1-15) + ?
-
?

Synonyms

Synonyms Comment Organism
Activated protein C
-
Mus musculus
Activated protein C
-
Homo sapiens
Activated protein C
-
Bos taurus
anticoagulant serine protease-activated protein C
-
Mus musculus
anticoagulant serine protease-activated protein C
-
Homo sapiens
anticoagulant serine protease-activated protein C
-
Bos taurus
APC
-
Mus musculus
APC
-
Homo sapiens
APC
-
Bos taurus
ghrelin endopeptidase
-
Mus musculus
ghrelin endopeptidase
-
Homo sapiens
ghrelin endopeptidase
-
Bos taurus

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
22
-
assay at room temperature Mus musculus
37
-
assay at Homo sapiens
37
-
assay at Bos taurus

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
8
-
assay at Mus musculus
8
-
assay at Bos taurus

General Information

General Information Comment Organism
additional information enzyme peptide mass fingerprinting analysis Mus musculus
physiological function the enzyme is a major regulator of blood coagulation by inactivating factors Va and VIIIa. O-ghrelin(15) has no effect Mus musculus
physiological function the enzyme is a major regulator of blood coagulation by inactivating factors Va and VIIIa. O-ghrelin(15) has no effect Homo sapiens
physiological function the enzyme is a major regulator of blood coagulation by inactivating factors Va and VIIIa. O-ghrelin(15) has no effect Bos taurus