Inhibitors | Comment | Organism | Structure |
---|---|---|---|
Zn2+ | Zn2+ enhances the binding of protein C/activated protein C to endothelial cell protein C receptor on endothelial cells by increasing the binding affinities. Zn2+ binding induces conformational changes in protein C/APC. Zn2+ binding to APC inhibits the amidolytic activity of APC, but the inhibition is reversed by Ca2+. Zn2+ increases the rate of APC generation on endothelial cells in the presence of physiological concentrations of Ca2+, but does not further enhance increased APC generation obtained in the presence of physiological concentrations of Mg2+ with Ca2+. Zn2+ has no effect on the anticoagulant activity of APC. Zn2+-mediated inhibition of APC amidolytic activity rather than the direct inhibition of protein C activation | Homo sapiens |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Ca2+ | the presence of Ca2+ is essential for Zn2+ to enhance protein C activation because Zn2+ alone fails to support protein C activation | Homo sapiens | |
Zn2+ | Zn2+ enhances the binding of protein C/activated protein C to endothelial cell protein C receptor on endothelial cells by increasing the binding affinities. Zn2+ binding induces conformational changes in protein C/APC. Zn2+ binding to APC inhibits the amidolytic activity of APC, but the inhibition is reversed by Ca2+. Zn2+ increases the rate of APC generation on endothelial cells in the presence of physiological concentrations of Ca2+, but does not further enhance increased APC generation obtained in the presence of physiological concentrations of Mg2+ with Ca2+. Zn2+ has no effect on the anticoagulant activity of APC. Zn2+ enhances APC-mediated activation of protease activated receptor 1 and p44/42 MAPK | Homo sapiens |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
Factor Va + H2O | Homo sapiens | - |
? | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Homo sapiens | - |
- |
- |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
commercial preparation | - |
Homo sapiens | - |
endothelial cell | primary human umbilical vein endothelial cells, HUVEC | Homo sapiens | - |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
Factor Va + H2O | - |
Homo sapiens | ? | - |
? | |
pyroGlu-Pro-Arg-4-nitroanilide + H2O | S-2366, APC is labeled with AF546 | Homo sapiens | pyroGlu-Pro-Arg + 4-nitroaniline | - |
? |
Synonyms | Comment | Organism |
---|---|---|
Activated protein C | - |
Homo sapiens |
APC | - |
Homo sapiens |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
37 | - |
assay at | Homo sapiens |
General Information | Comment | Organism |
---|---|---|
metabolism | the protein C pathway plays a key role in blood coagulation and inflammation | Homo sapiens |
additional information | APC generation is dependent on protein C binding to endothelial cell protein C receptor, EPCR, and thrombin binding to thrombomodulin | Homo sapiens |