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Literature summary for 3.4.21.69 extracted from

  • Yegneswaran, S.; Kojima, Y.; Nguyen, P.M.; Gale, A.J.; Heeb, M.J.; Griffin, J.H.
    Factor Va residues 311-325 represent an activated protein C binding region (2007), J. Biol. Chem., 282, 28353-28361.
    View publication on PubMed
Search for more literature for 3.4.21.69

Activating Compound

Activating Compound Comment Organism Structure
Phospholipids
-
 Homo sapiens

Cloned(Commentary)

Cloned (Comment) Organism
expression of wild-type an dmutant enzymes in HEK-293 cells Homo sapiens

Protein Variants

Protein Variants Comment Organism
S360A site-directed mutagenesis, the active site residue mutant shows no amidolytic activity Homo sapiens
S360C site-directed mutagenesis, the active site residue mutant shows no amidolytic activity Homo sapiens

Inhibitors

Inhibitors Comment Organism Structure
peptide VP311-325 a peptide comprising residues 311-325 in factor Va, potent inhibition of factor Va-dependent prothrombin activation by factor Xa in the absence of APC, the peptide also inhibits the inactivation of factor Va by APC by APC-dependent cleavage of both Arg506 and Arg306, thus inhibits cleavage of wild-type factor Va and of the mutant R306Q and R506Q, overview Homo sapiens

Metals/Ions

Metals/Ions Comment Organism Structure
Ca2+ required Homo sapiens
Mn2+
-
 Homo sapiens

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
active factor Va + H2O Homo sapiens proteolytic inactivation inactive factor V + ?
-
 ?

Organism

Organism UniProt Comment Textmining
Homo sapiens
-
-
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
active factor Va + H2O proteolytic inactivation Homo sapiens inactive factor V + ?
-
 ?
active factor Va + H2O proteolytic inactivation, cleavage of the heavy chain at Arg506, Arg306, and Arg679, factor Xa protects factor Va from inactivation by APC, interaction of factor Va and APC at residues 311-325 of the substrate Homo sapiens inactive factor V + ?
-
 ?
active factor Va mutant R306Q + H2O proteolytic inactivation, cleavage of the heavy chain at Arg506 and Arg679 Homo sapiens inactive factor V mutant R306Q + ?
-
 ?
active factor Va mutant R506Q + H2O proteolytic inactivation, cleavage of the heavy chain at Arg306 and Arg679 Homo sapiens inactive factor V mutant R506Q + ?
-
 ?
active factor Va mutant R679Q + H2O proteolytic inactivation, cleavage of the heavy chain at Arg306 and Arg506 Homo sapiens inactive factor V mutant R679Q + ?
-
 ?
Z-Gly-Gly-Arg-aminomethylcoumarin + H2O a fluorogenic substrate, S-2366 Homo sapiens ?
-
 ?

Synonyms

Synonyms Comment Organism
Activated protein C
-
 Homo sapiens
APC
-
 Homo sapiens

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
37
-
 assay at Homo sapiens

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7.4
-
 assay at Homo sapiens