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Literature summary for 3.4.21.69 extracted from

  • Yang, L.; Manithody, C.; Rezaie, A.R.
    Contribution of basic residues of the 70-80-loop to heparin binding and anticoagulant function of activated protein C (2002), Biochemistry, 41, 6149-6157.
    View publication on PubMed
Search for more literature for 3.4.21.69

Cloned(Commentary)

Cloned (Comment) Organism
protein C mutants prepared by PCR methods expressed in HEK293 cells Homo sapiens

Protein Variants

Protein Variants Comment Organism
K78A prepared by PCR methods Homo sapiens
R74A prepared by PCR methods Homo sapiens
R74E/R75E/K78E triple mutant, Gla-domainless form of APC Homo sapiens
R75A prepared by PCR methods Homo sapiens

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.131
-
 spectrozyme PCa pH 7.4, 37°C, mutant R74A Homo sapiens
0.133
-
 spectrozyme PCa pH 7.4, 37°C, mutant K78A Homo sapiens
0.146
-
 spectrozyme PCa pH 7.4, 37°C, mutant R75A Homo sapiens
0.164
-
 spectrozyme PCa pH 7.4, 37°C, wildtype enzyme, recombinant expressed Homo sapiens
0.177
-
 spectrozyme PCa pH 7.4, 37°C, wildtype plasma enzyme Homo sapiens

Metals/Ions

Metals/Ions Comment Organism Structure
Ca2+
-
 Homo sapiens

Organism

Organism UniProt Comment Textmining
Homo sapiens P04070 human
-

Purification (Commentary)

Purification (Comment) Organism
recombinant enzyme Homo sapiens

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
spectrozyme PCa + H2O
-
 Homo sapiens ?
-
 ?

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
44
-
 spectrozyme PCa pH 7.4, 37°C, wildtype plasma enzyme Homo sapiens
47
-
 spectrozyme PCa pH 7.4, 37°C, wildtype enzyme, recombinant expressed and mutant R74A Homo sapiens
48
-
 spectrozyme PCa pH 7.4, 37°C, mutant K78A and mutant R75A Homo sapiens