Application | Comment | Organism |
---|---|---|
medicine | recombinant human t-PA is extensively used as a therapeutic agent for the treatment of thrombotic diseases owing to its greater safety and efficiency compared with other plasminogen activators like urokinase and streptokinase. CERTS132A-based secretion engineering can be an effective strategy for enhancing recombinant enzyme production in CHO cells | Homo sapiens |
Cloned (Comment) | Organism |
---|---|
recombinant overexpression of the enzyme in CHO ATCC CRL-9606 cells and secretion to the cell culture medium, co-expression with CERT S132A, a mutant form of CERT that is resistant to phosphorylation, and XBP1s, method optimization and evaluation, overview. Overexpression of CERT S132A increases the specific productivity of t-PA-producing CHO cells up to 35%, while the heterologous expression of XBP1s does not affect the t-PA expression rate | Homo sapiens |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
extracellular | - |
Homo sapiens | - |
- |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
68000 | - |
x * 68000, recombinant enzyme, SDS-PAGE | Homo sapiens |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
plasminogen + H2O | Homo sapiens | activation | plasmin + ? | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Homo sapiens | P00750 | - |
- |
Source Tissue | Comment | Organism | Textmining |
---|
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
plasminogen + H2O | activation | Homo sapiens | plasmin + ? | - |
? |
Subunits | Comment | Organism |
---|---|---|
? | x * 68000, recombinant enzyme, SDS-PAGE | Homo sapiens |
Synonyms | Comment | Organism |
---|---|---|
t-PA | - |
Homo sapiens |
Tissue plasminogen activator | - |
Homo sapiens |
General Information | Comment | Organism |
---|---|---|
physiological function | the human tissue plasminogen activator is a serine protease known as the key component of the fibrinolytic system. t-PA converts the proenzyme plasminogen to plasmin, which in turn mediates the degradation of fibrin | Homo sapiens |